lon concentrations inside and outside of a typical mammalian cell are not the same. Which of the following ions have (has) a higher concentration outside than inside? Na+ and Ca2+ K+ and Na+ K+ and Cl- K+ OK+, Cl-, and Ca2+
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- Let’s say that the intracellular tonicity is 300 mosm. Checkmark the correct response to what would happen to the cell when it is placed in the following solutions prepared from sucrose (impermeable), NaCl (impermeable), and urea (permeable). Solution Nothing Swell Shrink 100 mM sucrose 200 mM NaCl 300 mM urea 300 mM NaCl 100 mM sucrose + 100 mM NaCl 100 mM ureaOne of the important uses of the Nernst equation is in describing the flow of ions across plasma membranes. Ions move under the influence of two forces: the concentration gradient (given in electrical units by the Nernst equation) and the electrical gradient (given by the membrane voltage). This is summarized by Ohms law: Ix=Gx(VmEx) which describes the movement of ion x across the membrane. I is the current in amperes (A); G is the conductance, a measure of the permeability of x, in Siemens (S), which is I/V;Vm is the membrane voltage; and Ex is the equilibrium potential of ion x. Not only does this equation tell how large the current is, but it also tells what direction the current is flowing. By convention, a negative value of the current represents either a positive ion entering the cell or a negative ion leaving the cell. The opposite is true of a positive value of the current. a. Using the following information, calculate the magnitude of Na [ Na+ ]0=145mM,[ Na+ ]i=15mM,Gna+=1nS,Vm=70mV b. Is Na+ entering or leaving the cell? c. Is Na+ moving with or against the concentration gradient? Is it moving with or against the electrical gradient?Liver cells are in contact with the blood and exchange a variety of substances with the blood plasma (the noncellular part of blood). The concentration of water is equal in the cytoplasm of liver cells and in the blood plasma. Explain this observation in terms of membrane permeability and transport mechanisms. Animal cells typically maintain a higher concentration of Na+ outside the cell and a higher concentration of K+ inside the cell via the Na+-K+ pump. The drug ouabain inhibits the activity of the Na+-K+ pump. A nerve cell is incubated in ouabain. Predict what will happen to the concentrations of Na+ and K+ inside and outside the nerve cell as a result.
- For most neurons, the extracellular concentration of chloride ions (Cl-) is 108 mM, whilethe intracellular concentration of Cl- is 5 mM.If the plasma membrane becomes more permeable to Cl-, would there be Clinflux or Cl- efflux at an RMP of -70 mV? Why?;, Consider a spherical cell at body temperature (37°C) that is 10 µm in radius. The concentration of ions inside and outside the cell are given below. Sodium and potassium are the only permeant ions, and the membrane contains proteins that pump in 3 sodium ions for every 2 potassium ions pumped out. [Na*] = ? [K*] = 119mM [Ci] = 8mM [A] = 121mM [Na*] = 120mM [K*] = ? [Cr] = 125MM [glu] = ? (a) Suppose the cell is in osmotic equilibrium, find the 3 unknown concentrations. (b) Suppose the membrane conductance to sodium is 5% that to potassium, find the resting membrane potential via circuit analysis. (c) What would the resting potential be if the membrane were instead permeable only to chloride ions? (d) Suppose the cell was suddenly placed in distilled water, what would happen to it? And, how much external pressure (positive or negative) would be needed to prevent this from happening?Sugars are transported in symport with Na+. Na+ upon some time accumulates causing the need to transport the Na+ back to outside the cell. How is this transport effected so that equilibrium concentrations of electrolytes are maintained in the cells, in accordance with transport in animal cells?
- Under which circumstance is the Goldman equation equivalent to the Nernst equation? O when the cell membrane is only permeable to 1 ion O when the concentrations of the ions are equal outside and inside of the cell when the cell membrane is equally permeable to all ions O when there is no charge on the membraneLeft Compartment Right Compartment 7 grams of Z per liter solution 4 grams of XY per liter of solution total volume = 1 liter total volume = 1 liter Solute Z has a molecular weight of 20 (non-dissociable) Solute XY has a molecular weight of 10 (dissociates into X+ and Y-) If X+ and Y- are ions, which are permeable through the membrane, and Z is not, what will happen and why?Salt is being used to preserve salmon. You need to determine the time required for the salt concentration to reach a known value at the center of the salmon. The concentration of salt at the surface (CAS) is known, and so is the initial concentration (CAO) in the salmon muscle. You also know the mass diffusivity, Ds-s, of salt in salmon muscle. What type of system is this? O Steady State Diffusionn, no reaction O Unsteady State Diffusion O Steady State Diffusion, with reaction O Pseudosteady State O A through stagnate B
- In the Nernst equation [V = 62 log10 (Co / Ci)], the term Co represents: cell bio the intracellular concentration of calcium the extracellular concentration of potassium the extracellular concentration of sodium the intracellular concentration of potassium the membrane potential (in millivolts)The sodium-calcium-exchanger (NCX) protein is an antiport protein that is located in the membranes of cardiac muscle cells in humans as well as other cells. Antiport proteins transport two different solutes in opposite directions. The sodium-calcium-exchanger protein functions by secondary active transport and requires the sodium-potassium pump in order to function. The sodium-potassium pump functions by primary active transport and transports sodium ions from the intracellular fluid to the extracellular fluid. • Describe how the sodium-potassium pump provides energy for the function of the sodium-calcium-exchanger. Considering that the NCX protein is an antiport protein, describe the concentration gradient of calcium on both sides of the cell membrane. Describe the direction that the calcium ions are transported.The parasite Trypanosoma brucei, which causes sleeping sickness, uses proline as an energy source during one stage of its life cycle. The properties of this proline-specific transporter were investigated in a series of experiments. L-hydroxyproline inhibited the transporter. D-proline did not inhibit the transporter. Na*, K*, and pH changes did not affect the transporter. Which of the following is a reasonable hypothesis based on this data? The proline transporter is a Na+-K+ ion pump. The proline transporter cleaves amide bonds. The proline transporter is stereospecific. The proline transporter requires metal-ion catalysis. The proline transporter has an essential ionizable histidine residue,