Electrophoretic Analysis of DNA via Agarose gel Electrophoresis Lane 1 Lane 2 Lane 3Sample2:PBR3222Base PairsDNA Mass(ng/Sul)Sample1:TUC191988°88884,0003.0002.5002,000DNA34ladder201,50092-1.000237503050045-2501% TAE agarose gelResuits of DNA gel electrophoresis(after editing brightaess.DNA ladder that we used as marker in Lane no 1Lane 1 Lane 2 Lane 3Base PairsDNA Mass(ng/5ul)Sample2:PBR32221988°8.888Sample1:TUC194,0003.0002,5002,00034201,500DNA92-1,000ladder23-75030500452501% TAE agarose gelResults of DNA gei|electrophoresis (before editing brightness)DNA ladder that we used as marker in Lane no 1Determine the size (base pairs) for sample 1 and sample 2 (refer to the DNA ladderfigure and compare with the results to determine the size).

Agarose gel electrophoresis is a technique which is used to separate the DNA on the basis of their…

Lane 1 Lane 2 Lane 3 Sample2: PBR3222 DNA Mass Base Pairs (ng/Sul) Sample1: TUC19 1988 :8888 4.000 3.000 2.500 2,000 DNA 34 ladder 20 1,500 1,000 23 750 30 -s00 45 -250 1% TAE agarose gel Results of DNA gel electrophoresis (after editing brightness. DNA ladder that we used as marker in Lane no 1 Lane 1 Lane 2 Lane 3 Base Pairs DNA Mass (ng/Sul) Sample2: PBR3222 Sample1: TUC19 988° 888 4,000 3.000 2.500 2.000 20 1,500 DNA 92 1,000 ladder 23 750 30 500 250 1% TAE agarose gel Results of DNA gel electrophoresis (before editing brightness) DNA ladder that we uzed as marker in Lane no 1 Determine the size (base pairs) for sample 1 and sample 2 (refer to the DNA ladder figure and compare with the results to determine the size).

Lane 1 Lane 2 Lane 3 Sample2: PBR3222 DNA Mass Base Pairs (ng/Sul) Sample1: TUC19 1988 :8888 4.000 3.000 2.500 2,000 DNA 34 ladder 20 1,500 1,000 23 750 30 -s00 45 -250 1% TAE agarose gel Results of DNA gel electrophoresis (after editing brightness. DNA ladder that we used as marker in Lane no 1 Lane 1 Lane 2 Lane 3 Base Pairs DNA Mass (ng/Sul) Sample2: PBR3222 Sample1: TUC19 988° 888 4,000 3.000 2.500 2.000 20 1,500 DNA 92 1,000 ladder 23 750 30 500 250 1% TAE agarose gel Results of DNA gel electrophoresis (before editing brightness) DNA ladder that we uzed as marker in Lane no 1 Determine the size (base pairs) for sample 1 and sample 2 (refer to the DNA ladder figure and compare with the results to determine the size).

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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BamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…
Lane 1 Lane 2 Lane 3 Sample2: PBR3222 Base Pairs DNA Mass (ng/Sul) Sample1: TUC19 2888 4.000 3,000 2,500 2,000 DNA 34 ladder 1,500 92 1,000 23 750 30 -500 45 250 1% TAE agarose gel Resuits of DNA gel electrophoresis (after editing brightnessm DNA ladder that we used as marker in Lane no 1 Lane 1 Lane 2 Lane 3 Base Pairs DNA Mass (ng/5ul) Sample2: PBR3222 1988° Sample1: TUC19 4,000 3.000 2.500 -2.000 1,500 34 20 DNA 1,000 92 ladder 23 750 30 500 45 250 1% TAE agarose gel Resuits of DNA gel| electrophoresis (before editing brightness) DNA ladder that we used as marker in Lane no ! Determine whether sample 1 and sample 2 are plasmid OR genomic DNA, justify your answer
t (5 a 1 dao) - Google Chrome edu.om/mod/quiz/attempt.php?attempt=2334320&cmid=1127808 SQU E-LEARNING SYSTEM (ACAI الوقت المتبقي 0.175mm .e O In the DNA extraction experiment, the separation of DNA from its packaging protein can be achieved by the addition of Detergent aO 1.00 Salt b O هذا السؤال Ethyl alcohol cO Papain powder .d O Cold water Le O In paramecium, osmoregulation occurs through 3 Activate Windowssk Macronucleus Goaotings to acttivate do nere to search 6. 70°F Mostly sunny 9:05 ENG 12/18 f2 O f3 O f4 O f6 O f8 end f7 0 F10 insert f9 D F12 T num IA break @ %24 & 8 2 3 r 4 5 6 7 8 A 9 9 R Y ! U S F G J K +|| 回 Q 以 4
ersonal/eenongen_my_tnstate_edu/_layouts/15/doc.aspx?sourcedoc={a6b083c9-a226-4c31... ☆ Search (Option + Q) Review View Help Picture Editing A В ... During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2 6. 9. 10 IV 13 14 Which of the following best describes the trait in the pedigree? Question 2 options: X-linked dominant X-linked recessive autosomal domiant autosomal recessive ON
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
A: Lambda DNA/Hindlll Marker, 2 B: GeneRuler" 1 kb DNA Ladder tp no0.5 O'GeneRuler" 1 kb DNA Ladder, ready-to-use bp ng/0.5 pg 23130 2384 07 W1 19.4 676 13.5 4961 450 9416 6557 9.0 564 5.8 1500 25.0 5.0 12 750 1000 60.0 12.0 25.0 5.0 125 13 0.3 500 25.0 5.0 250 25.0 5.0 0.5pon, Bom lngth g 1XTAE, 7Vom 45 mi Range 8 fragments on bp: 23130, 9416, 6557, 4361 2322, 2027,.564, 125 0.5 pgtne, 8 cm kength gel IIXTAE, 7VRm 45 min Which ladder would be the BEST to use if you had a sample that should measure both 1010 bp and 2027 bp? Tapito" LE GO Agune (RO1)
A sample of DNA with the sequence 5'- CTC GAG CGA AGC TCA ACC-3' he was obtained as a dry solid. The sample was dissolved in 1000 µl of deionized water ane mixed well. Ten microliters of the dissolved sample was then transferred to a new sampie tube and mixed with 990 ul of water, The dilute sample had A260 = 0.156. What was the concentration of the original sample (the solid dissolved in 1000 ul of deionized water)
Please I want the answer of (B) and (C) Task 1. Your graduate advisor asks you to amplify the following sequence of DNA by PCR: 5’-ATACGCATTCGGACCAGGTCCTAA-3’ 3’-TATGCGTAAGCCTGGTCCAGGATT-5’ a. To ensure that the entire sequence above is amplified, what 6-nucleotide DNA primers should you add to your PCR mix? You order the primers listed above, but instead receive the following set of primers: 5’-CGCATT-3’ 5’-GGACCT-3’ b. What portion of the double stranded DNA molecule will be amplified after 10 rounds of PCR? Your labmate attempts to rescue your PCR reaction by providing you with the following set of primers: 5’-ATACGC-3’ 5’-TCCTAA-3’ c. What is the result of running the PCR reaction with your labmate’s primers? How many double stranded molecules of DNA will result from 10 rounds of amplification?
Aa Po AaBbCcDc AaBbCcDdEe AaBbCcDdE AaBbCcDdE AaBbCcDdE BIUVab x, x A ev A 三==|這、|の Caption Emphasis Heading 1 Heading 3 Heading 4 Style Pane (a) Complete the table, using a letter D, R or B, to show whether each statement applies to: Fo DNA only (D) TA2G – Completed forms must be available for Open Awards external moderation purposes. Page 6 of 15 > T | RNA only (R) Both DNA and RNA (B) Statement D or R or B Contains thymine Contains ribose Consists of two chains connected to each other with hydrogen bonds Has a sugar-phosphate backbone Has four different nitrogenous bases Contains a pentose sugar Is found in the nucleus and cytoplasm English (United Kingdom) Focus 目民 2490 words +1
Table I CACGT A GA CTGAGG ACTC CACGTAGACTGAG G ACAC Wild-type beta-globin gene fragment Sickle-cell beta-globin gene fragment > Circle the mutation in DNA of the sickle-cell beta-globin gene fragment Compare fragments of DNA the wild-type and mutant beta-globin genes in the Table I above, what are the similarities and differences you observe?
HindlII ECORI ECORV BamHI 379 Sall Pstl. tet amp Plasmid PBR322 1000 4361 bp ori Ndel If you were going to use this vector to clone a foreign fragment of DNA, what two restriction enzymes would you use to cut the vector, so you could make a recombinant plasmid that has AmpR and KanR (Kanamycin resistance), but No TetR. Assume the insert DNA does NOT have a Tet resistance gene but does have a Kanamycin resistance gene. O EcoR1 and Hindll O Pst1 and Sal1 O Pst1 and Nde1 O EcoR1 and Nde1 f6
B 6000 5100 Number of Fragment(s) 4000 100 pX 6000 bp 3000 A A 1500 2000 A B 10. Based on the restriction map of the above plasmid, determine the number of DNA fragment(s) and the size(s) of the fragment(s). Digestion with Enzyme A Enzyme B Enzyme C Enzyme A + B Enzymes A + C Enzyme B+ C Enzyme A + B + C Fragment size(s) in base pairs