An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: м 1 M 2 3 4 ng/0.5 µg bp 20 5000 20 4000 40 3000 20 2000 20 1000 60 500 20 100 Determine the amount of the total DNA ladder added in the gel in terms of ug

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Chapter1: Biochemistry: An Evolving Science
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An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded
into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading
buffer were added into the gel.
Shown below is the AGE profile and the needed marker details where M is the MW
marker while numbers 1-4 indicate the lanes with the DNA samples:
м 1
M
2
3
4
ng/0.5 µg
bp
20
5000
20
4000
40
3000
20
2000
20
1000
60
500
20
100
Determine the amount of the total DNA ladder added in the gel in terms of ug
Transcribed Image Text:An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: м 1 M 2 3 4 ng/0.5 µg bp 20 5000 20 4000 40 3000 20 2000 20 1000 60 500 20 100 Determine the amount of the total DNA ladder added in the gel in terms of ug
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