Why is Taq polymerase used in a polymerase chain reaction (PCR)? O Unlike other DNA polymerases, Taq polymerase is heat stable and survives the 94 degree denaturation step in PCR. Taq polymerase is more efficient than other DNA polymerases. O Taq polymerase can produce DNA from an RNA template, unlike other DNA polymerases. O Taq polymerase has proofreading activity and thus makes fewer errors than other DNA polymerases. Question 44 Which of the following statements about the differences between prokaryotic and eukaryotic genomes is FALSE? O Prokaryotic genomes have centromeres, whereas eukaryotic genomes do not. O Eukaryotic genomes have telomeres, whereas prokaryotic genomes do not. Prokaryotic genomes have one origin of replication, whereas eukaryotic genomes have multiple origins of replication. O Prokaryotic genomes are less complex than eukaryotic genomes.

In a Sanger sequencing reaction, the template DNA is shown below, with the short sequence on top as the primer: 5' ATG 3' 3' TACACTATCGCA 5' A reaction is carried out with the following components: primer, template, DNA polymerase, dNTPs, and ddGTP. Write out all the possible PCR products (label 5' and 3' end).

Can you please help explain which best describes the role of topoisomerase?

DNA polymerases can only add new dNTPs to a primer strand that is hydrogen-bonded to the template. True or false

Translesion synthesis (TLS) polymerase zeta (ζ) is capable of accurately reading through 6,4-photoproducts (cytotoxic damage). If this protein is non-functional:       a different TLS polymerase will accurately read through the DNA lesion.   a different TLS polymerase will inaccurately read through the DNA lesion resulting in increased mutational frequency.   the replicative polymerase will inaccurately read through the DNA damage.   DNA polymerase alpha (α) will be recruited to synthesize a primer across the DNA damage.   the DNA lesion cannot be bypassed and will result in double stranded DNA breaks.

The antibiotic Cipro (ciprofloxacin) inhibits DNA gyrase found in bacteria. Why would this stop bacteria from dividing? Group of answer choices 1. DNA polymerase I could not remove RNA primers 2. Helicase would not be able to fully unwind the replication bubble due to stress in the DNA 3. Okazaki fragments would come off of the newly replicated DNA dnaA would not bind to the OriC 4. DNA Polymerase III would be halted on only the lagging strand