Based on the quantitation data, please indicate how you would dilute the following samples. Fill in each blank with the appropriate information. Item 1: DNA concentration - 22.6 ng/uL Dilution factor - 1: Add ul DNA extract to ul buffer Final dilution concentration = ng/ul Item 4: DNA concentration - 3.75 ng/uL Dilution factor - 1: Add uL DNA extract to uL buffer Final dilution concentration = ng/uL

Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be closest to the positive or negative electrode?

A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.

tudent forgot to add GelRed in to the gel mixture when he prepared the Agarose Gel. What will be the problem caused by his mistake?     A) He will not be able to securely deposit his samples in the wells, the samples will disperse into the buffer     B) The student will have a hard time visualizing how far his samples have traveled in the gel to disconnect the power supply in time     C) The student will not be able to visualize the DNA under the UV light     D) The student 's DNA will get degraded during the process of electrophoresis     E) More than one of the answer choices are correct

How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines)  .

How many microliters of pGLO plasmid will you need for a PCR reaction is you need 200 nanograms and the plasmid concentration is 0.05 micrograms/microliter?

Which of the following gels is the preferred choice for separation of DNA fragments? Agarose Polyacrylamide SDS PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis) Agar

The table shows the presence/absence and size of DNA bands of known samples using gel electrophoresis. Explain conclusions you can draw from the data in the table.

Consider the four extraction/purification methods . a. Which extraction method would you use for a touch DNA sample? Explain your reasoning.b. Which extraction method would you use for a sample containing a mixture of primarily non-human and some human DNA? INFO: the 4 extraction methods are QIAamp manual extraction, QIAcube semi-automated extraction, DNA IQ extraction, or FTA punch purification

3) Were all of the conditions of a standardized Kirby-Bauer test met as you performed this assay? If not, which were not? 4) What is the significance of colonies that develop within otherwise clear zones of inhibition? If the laboratory report for one of your patients indicated colonies within the zone, what concerns would you have for your patient?