Based on the quantitation data, please indicate how you woulddilute the following samples. Fill in each blank with the appropriateinformation.Item 1:DNA concentration - 22.6 ng/uLDilution factor - 1:AdduL DNA extracttouL bufferFinal dilution concentration =ng/ulItem 4:DNA concentration - 3.75 ng/uLDilution factor - 1:AdduL DNA extracttouL bufferFinal dilution concentration =ng/ul

DNA shows maximum absorbance at the wavelength of 260 nm. Hence the concentration of DNA in solution…

Answered: Based on the quantitation data, please…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

In your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final volume will be 20 ?μl. How much of each of the following reagents will you use to obtain the indicated working concentrations? 10x restriction buffer >> working concentration 1x 1 mg/mL bovine serum albumin (BSA) >> working concentration 100?μg/mL
complete the table below
Which of the following statements is TRUE for DNA extraction? DNA yield and purity are inversely proportional. The same protocol can be used for isolating bacterial gDNA and pDNA. OCTAB extraction is only effective for plant cells. extraction is safe routine Phenol-chloroform protocol.
Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Total μL µL/Rxn (total per tube) Final Number of Stock Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 µM MgCl2 Forward 24 mM 2.5 mM 2 μΜ 0.1 μΜ primer Reverse 2 μΜ 0.1 μΜ primer Taq polymerase DNA 5 U/uL 0.03 U/ µL 1 μL 60 μL Total volume of the entire reaction (µL)
What is the principle of the gel electrophoresis separation technique?
Three pieces of cut DNA were isolated using gel electrophoresis.The DNA that is the longest will be closest to the positive or negative electrode?
A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
DNA Extraction and Characterization from Shrimp Describe the resulting gel, assess the purity of your sample. Show calibration curve. Calculate the MW of the sample, and compare to literature. Rationalize possible discrepancies.
Which of the following conditions will affect the specificity of primer annealing? You may choose more than one answer. Annealing temperature Denaturation temperature Polymerization time _ [MgCl2]
Briefly describe the function of each of the following components of the DNA extraction protocol. Please consult the practical manual. (Students taking the online alternative, derive these steps from the practical manual and BI101JC_Biochemistry Practical DNA PROTEIN, or from the Connect Virtual Lab on DNA Extraction). i. Heat ii. Protease Solution (protein-digesting enzymes in meat tenderizer) iii. Detergent (emulsifier) iv. Salt v. Ethanol
tudent forgot to add GelRed in to the gel mixture when he prepared the Agarose Gel. What will be the problem caused by his mistake? A) He will not be able to securely deposit his samples in the wells, the samples will disperse into the buffer B) The student will have a hard time visualizing how far his samples have traveled in the gel to disconnect the power supply in time C) The student will not be able to visualize the DNA under the UV light D) The student 's DNA will get degraded during the process of electrophoresis E) More than one of the answer choices are correct
What is the difference between a random sample and a composite sample? When would each be used?

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS