The human genome is made from more than 3 billion nucleotides.  How many sites (loci) are characterized (genotyped) in typical forensic DNA profiling tests performed as part of an investigation?     1   13   1,000   1,000,000

Humans have very similar DNA sequences, with approximately 999/1000 letters being ide One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more people

What is DNA fingerprinting? Mention its application.

. Lane 1 represents DNA taken from the murdered victim.  Lane 2 through 5 are DNA samples taken from suspects in the crime.  Lane 6 is from a bloodstain found near the victim.   Q6. Why is it important to include the DNA taken from the murdered victim (Lane 1) in this DNA analysis?

Many times a forensic scientist has only a single hair or a single drop of blood for analysis. The amount of DNA in this kind of material is very tiny, and is not enough to use for comparison. In other words, running a single experiment to compare the DNA in a drop of blood with several suspects would use up all of the DNA in that drop of blood. This could be a serious problem if none of the suspects provided a match. How would a forensic scientist increase the amount of DNA available to them, starting with the DNA that would be found in their crime scene sample, such as a drop of blood from a suspect? PCR RFLPS Gel electrophoresis STRS

Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were left behind, from which DNA was extracted. An individual was caught with all five of the stolen textbooks in his bag. He claims his friend gave them to him.   You decide to amplify two regions of the DNA (two loci, plural of locus) and digest the samples, and get the following result on the electrophoresis gel.L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene (Note: assume bands that are close in distance traveled are the same length. Higher concentrations of DNA can make a band appear to travel farther.)   Which band patterns are similar?

Which of the following statements is/are true? Choose only the best answer. Repetitive DNA can influence the appearance of an organism. Simple sequence DNA, transposable elements, and large-segment duplications are examples of repetitive DNA. Repetitive DNA can be useful in genetic profiling. All three of the other statements are true.

Which of the following criteria do you feel is the most important one to optimize for a DNA profiling methods?   Speed   Discriminating power   Sensitivity   Cost   False Positive Rate

How to find any evidence of cantamination or degradation of DNA in the DNA profiles you examined?   how important is the detabase that is used to determine allele frequencies in DNA profiling cases

BamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…