The human genome is made from more than 3 billion nucleotides.  How many sites (loci) are characterized (genotyped) in typical forensic DNA profiling tests performed as part of an investigation?     1   13   1,000   1,000,000

Q: There are two missing fishermen. Multiple human remains were found in the shark. Are the remains…

A: To determine whether the remains (Samples 01, 02, and 03) are from one person, two people, or three…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: if 3ul of DNA ladder is added on the gel AGE which has a concentration of 100ng/ ul. Then it means :…

Q: What would be the final primer concentration if 0.5 ul of 10 uM primers were ad a PCR reaction with…

A: Introduction: A primer is a short strand of DNA or RNA that is usually about 18-22 bases and serves…

Q: A ssDNA solution is diluted by taking 3uL of a stock solution, which is made up to 60uL with water.…

A: Beer-Lambert law relates the attenuation of light to the properties of the material through which…

Q: Given heart rate = 72 bpm, stroke volume = 70 mL, calculate cardiac output.

A: Stroke volume: All the blood from body enters into right atrium from where it is pumped into right…

Q: Discuss the regulation of cardiac output during exercise.

A: The cells are the primary unit of life. The cells form the tissues which collectively form an organ.…

Q: hat are the steps in creating a dna profile

A: DNA profiling is also known as DNA fingerprinting. It is often used to identify the origin and…

Q: If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR…

A: PCR(polymerase chain reaction) is a molecular method to make multiple copies(amplify) of a small…

Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…

A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…

Q: bp 6000 5000 2000 1000 500 250 100 50 M 2.35u 4.26 7.88 9.47 10.68 12.27 Dye front: 16u the AGE run…

A: *Agarose gel electrophoresis is used to separate a mixed macromolecule like DNA or proteins in a…

Q: The profile includes 4 STR loci. (D8S1179, D21S11, D7S820, CSF1PO) At how many of these is the…

A: Short Tandem Repeat Analysis STR analysis is a molecular technique by which short tandem repeats…

Q: Write an analysis of the results, interpreting what you see in the smaller gell focusing on 6A, 6B,…

A: DNA fingerprinting is a technique which enables us to identify an individual via his unique Genome…

Q: The figure below represents the size of various SSRS that are used for forensic analysis. The bars…

A: vWA produce 200bp in PCR . All others produce less or more than 200 bp in PCR.  Here SSR means is…

Q: During gel electrophoresis, DNA fragments are separated such that the longest DNA samplas migrate…

A: It is false   The true statement is- during gel electrophoresis DNA fragment are separated such that…

Q: Explain the principles of DNA migration of sample A in the electrophoresis experiment

A: Electrophoresis is a separation technique that was used to separate the mixture of molecules from a…

Q: What does the alcohol do in this experiment? Why does the DNA rise to the top after adding alcohol?…

A: Extraction of DNA from fruits.

Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…

A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: DNA QUANTITATION Consult related references (textbooks or research articles) and list possible…

A: DNA quantitation refers to the process of measuring the amount of DNA present in a given sample.…

Q: Estimate the fragment sizes (bp) of the DNA bands from each sample lane

A: Introduction: In the gel electrophoresis, agarose derived from red seaweed acts as a sieve for…

Q: Should DNA phenotyping be used to identify or convict a suspect?

A: The DNA (deoxyribonucleic acid) is the hereditary unit of an organism. It consists of a specific…

Q: Determine whether sample 1 and sample 2 are plasmid OR genomic DNA, justify your answer

A: By observing the bands of the two samples; we can say that sample 1: Lane 2: PBR3222 : A clear and…

Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: the hurdles that must be cleared before a new DNA profiling methodology can be used is....…

A: Option (a),(b),(c),(d) are correct. a) Only one-tenth of 1% of human DNA differs from one individual…

Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…

A: Agarose gel  electrophoresis is an important technique used in molecular biology research. In case…

Q: What is the concentration of a double stranded DNA sample with the following values? A260 - 1.068…

A: dsDNA Nucleic acid concentration can be calculated by UV spectrophotometry efficiently by utilizing…

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: Mother = 2) Child ||| 16. 'Dad' 1 'Dad' 2 || 9) FAC 9) 'Dad' 3 Source:…

A: DNA fingerprinting or profiling refers to any DNA-based technique that identifies DNA from a…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…

A: PCR stands for polymerase chain reaction.

Q: Lane 1 Lane 2 Lane 3 Sample2: PBR3222 DNA Mass Base Pairs (ng/Sul) Sample1: TUC19 1988 :8888 4.000…

A: Agarose gel electrophoresis is a technique which is used to separate the DNA on the basis of their…

Q: Describe two methods in performing a DNA analysis, its possibleapplications, and how it can be so…

A: DNA (Deoxyribonucleic acid) analysis is a method of determining the characteristics of the…

Q: What is the biological and non-biological samples used in forensic DNA investigations?

A: Chromosomes are carrier of deoxyribonucleic acid (DNA). DNA is the genetic material. Each cell has…

Q: Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were…

A: Most likely the culprit is suspect 1. This is so as the banding pattern of suspect 1 is matching…

Q: The table below shows the percentage of the nitrogenous bases present in each sample. Which of the…

A: The nitrogenous bases are the part of nucleotide the nucleic acid. A, T, G and C are four…

Q: sample containing at least one molecule of DNA serves as the starting material for PCR.  True…

A: PCR or polymerase chain reaction is routinely used in molecular biology laboratories that ultimately…

Q: In typing DNA from a sample found at a crime scene, how can a DNA mismatch prove that a suspect is…

A: DNA typing is also known as DNA fingerprinting. It is a method used to distinguish between…

Q: A 100ml dsDNA solution prepared via 1:20 dilution was taken for spectrometry analysis. The A260 and…

A: The objective of the question is to calculate the total DNA yield of the solution in micrograms. The…

Q: hich of the following ARE part of a typical PCR reaction mixture?     DNA ligase     dNTPs (mix…

A: Ans-  dNTPs(mix ofdeoxynucleoside tri-phosphates )

Q: STR #1: D5S818 allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' allele 2:…

A: DNA profiling is a laboratory forensic technique used to obtain the specific pattern of DNA from a…

Q: Answer choices : 0.3% gel OR 1.0% gel Explanation Choices : Agarose makes smaller pore sizes which…

A: Electrophoresis is an analytical technique that separates the biomolecule on the gel matrix based on…

Q: three criteria are DNA profiling methods evaluated by......   Speed, discriminating power and…

A: what is DNA profiling DNA profiling (also known as DNA fingerprinting) is the process of determining…

Q: To ensure a conviction, DNA crime labs use CODIS standards. Instead of 1 tandem repeat, how many…

A: Forensic science is concerned with the collecting and examination of physical evidence as well as…

Q: The sample furthest to the left contains the DNA size standards. What is the purpose of this sample?…

A: Gel electrophoresis is an analytical technique in which DNA fragments in a sample are separated…

Q: DNA adducts are a form of DNA damage caused by covalent attachment of a chemical moiety (like those…

A: DNA It is a nucleic acid having two polynucleotide chains wound around each other in a double helix…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.