B: Lambda DNA/Hindlll Marker, 2 GeneRuler" 1 kb DNA Ladder O'GeneRuler" 1 kb DNA Ladder, ready-to-use bp ng 0.5 pg 23130 2384 77 9416 W.1 19.4 -6557 4361 45.0 676 13.5 9.0 1500 25.0 564 .B 12 1000 60.0 12.0 750 25.0 50 125 13 03 500 25.0 5.0 250 25.0 5.0 05 poh Bom kngth g 1XTAE, 7Vom 45 m Range 8 fragments on b: 23130, 9416, 6557, 4361 2322, 2027, 564, 125 05 pgtine 8 cm kngth gl. |IXTAE. 7VRm 45 măn Which ladder would be the BEST to use if you had a sample that should measure both 1010 bp and 2027 bp? OA Taptso LE GO Agne RO1) 7 TopViLE GQ Agrose R0491)

y Cours S DIOE The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). Kpn I Sall Eco RI Pst Bam HI Xho Bam HI Pst i Eco RI Kon I 100 200 300 400 500 B00 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 How many total restriction fragments will result if you complete digest the DNA fragment above with Sal I? cross out a. 1 cross out b. 2 cross out O c. 3 cross out d. 4 cross out e. 5 cross out O f. 6 cross out g. 7 cross out Oh. 8 The following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). t of Sal Eco RI Pat Xhol Bam HI Psti Eco RI Koni Kpn i Bam H

What will be the outcome for any resulting protein from this this DNA segment  

During horizontal gene transfer, all of the following can be transferred EXCEPT (which statement is false?): O donor transposon DNA O recipient DNA O donor plasmid DNA O donor chromosomal DNA prt sc F10 home F9

Briefly explain how Genome Wide Association Studies (GWAS) are done. Then, using this example of a Manhattan Plot explain what each of the X-axis and the Y-axis signifies in a GWAS.

The X's correspond to the missing information. You need to determine what those X's represent DNA STRAND: XXX-CCC-GGG-GCG-XXX-XXX-XXX-GCC-ATA-TTA-XXX RNA STRAND: XXX-XXX-XXX-XXX-XXX-XXX-XXX-XXX-XXX-XXX-XXX PROTEIN: Met - X - X-X - Thr - Val - Glu-X-X-X - Trp To be clear, your answer is just the 3 sequences above without the X's (the highlighted yellow parts). There may be more than 1 correct answer for some parts of this assignment. Any answer your choose is fine. If you are unclear on how there could be more than 1 correct answer, review the lecture related to protein translation. Provide me with the: 1. Complete DNA sequence of 33 nucleotides 2. Complete RNA sequence of 33 nucleotides 3. 11 amino acids

12

SOD1 vs RM95 – 20% recombinant TGLA57 vs UMPS – 21% recombinant UMPS vs CRYG8- 17% Recombinant RM95 vs UMPS 50% - recombinant TGLA vs CRYG8 – 38% recombinant SOD1 vs TGLA57 – 49% recombinant RM95 vs TGLA 57 – 29% recombinant SOD1 vs UMPS – 50% recombinant what is the order of the genes on the chromosome and what is there distance in cM

A DNA fragment of interest is inserted in the BamHI site of plasmid PBR322.The recombinants will be: HindIII ECORI EcoRV BamHI 439 0 29 is 4000 375 Sall PstI, 651 tet amp 1000 PBR322 4361 bp 3000 ori 2000 2295 Ndel A. Resistant to amp an tet B. None of the other options, Xgal is required for selection O C. xensitive to amp, resistant to tet None of the other options, Xgal is required for selection O D. Sensitive to tet, resistant amp O E. Sensitive to amp and tet