In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.
In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.
Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter11: Filtration
Section: Chapter Questions
Problem 5RQ
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In sample preparation for electron microscopy, arrange the following steps in correct order
A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations.
B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol.
C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components.
D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons.
E. Allow the embedding medium to seep into the interstices of the tissue.
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