You are given a suspension of purified mitochondria that are known to have adequate reserves of NAD+, FAD, and ADP/inorganic phosphate. Assume that the mitochondrial preparation you are working with is free of cytosolic components (enzymes and metabolites) and assume that all the compounds listed below can cross both mitochondrial membranes, When glucose is added to this suspension: a. What is the effect on oxygen consumption by the purified mitochondria? b. Explain your answer above. When citrate is added to this suspension: c. What is the effect on oxygen consumption by the purified mitochondria? d. Explain your answer above.

How many molecules of ATP are generated by the complete mitochondrial beta oxidation of the unsaturated fatty acid nervonic acid (24:1), also known as 15-tertrasenoic acid (ie, it has a cis-delta 15 double bond). Which enzyme is required in the beta oxidation of nervonic acid that is not required for beta oxidation of lignoceric acid? and what reaction does this enzyme catalyze?

Calcium is an important regulator of the citric acid cycle. Calcium is transported across the mitochondrial inner membrane by a Ca²- 2+ uniporter that is driven by the negative potential inside the matrix. Part A Assuming a membrane potential across the inner membrane of 172 mV (inside negative), calculate the ratio of the [Ca2+] in the matrix to that in the cytoplasm ([Ca2+]m/[Ca²+]c) that would exist at equilibrium (i.e., AG = 0). Express your answer using two significant figures. [Ca2+]m [Ca2+] = ΤΟ ΑΣΦ Submit Request Answer Part B ? Cytoplasmic [Ca2+] is on the order of 10-7 M in a healthy cell. Based on your answer in Part A, calculate the [Ca2+] that would exist in the matrix at equilibrium. Express your answer to two significant figures and include the appropriate units. ☐ μÅ ? Value Units

Aconitase catalyzes the conversion of Citrate to Isocitrate in the TCA cycle. The standard free energy change (AG°) for this reaction is +6.7 kJ/mol. The observed free energy change (AG) for the reaction in pig heart mitochondria is +0.8 kJ/mol. The ratio of [isocitrate]/[citrate] in these mitochondria is: Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a 10 b. 1 100 0.01 e f 0.33 0.10 h 0.25 i 2