Hormone Concentration (nM) Protein 1 Protein 2 Protein 3 0.2 0.048 0.29 0.17 0.5 0.11 0.5 0.33 1 0.2 0.67 0.5 4 0.5 0.89 0.9 10 0.71 0.95 0.91 20 0.83 0.97 0.95 50 0.93 0.99 0.98
Q: Create a graph illustrating the activity of an enzyme or rate of reaction with the different factors...
A: Rate of reaction is the amount of product formed by the enzyme per unit time . Thisbis expressed in ...
Q: The melanocyte-stimulating hormone a-melanotropin has the following sequence: Ser-Tyr-Ser-Met-Glu-Hi...
A: Aminoacids are the building blocks of proteins that are linked by peptide bond. The sequence of amin...
Q: Substrates and reactive groups in an enzyme’s active site must be precisely aligned in order for a p...
A: Hi, First I would like to thank for submitting a question. As you have asked multiple question here ...
Q: How do enzymes function? What is the lock-and-key model and “induced-fit” in relation to its functio...
A: Enzymes are biological catalysts that help processes go faster. They are unique to their environment...
Q: 1. To which class does each enzyme belong? Explain your answers. a.) pyruvate decarboxylase ...
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that o...
Q: Determine the type of inhibition by AMP? Explain
A: Inhibitors are substances which inhibit the enzyme activity by binding to the active site or alloste...
Q: How did the scientists measure the exact amount of unfolded proteins at each temperature to determin...
A: The folding and unfolding of proteins is still a mystery. However, the unfolding dynamics of the pro...
Q: 1 Give the systematic names and the first three digits in the E.C. classifications of the enzymes ca...
A: Note : Hi ! Thank you for the question. We are authorized to answer three subparts at a time. Since ...
Q: which functional group remains charged at pH of 9. „NH2 H2N COOH
A: The pKa of the ionizable groups on an amino acid determines the net charge of the amino acid. Since ...
Q: 6. Amino acid alanine containing NE isotope in a-amino group was administrated to mouse with food. I...
A: The use of radioactive labelling of particular atoms have often been used to determine metabolic pat...
Q: 1. Which of these activities occur in ER? You can select more than one. Transcription Translation pr...
A:
Q: what type of inhibition is shown and where does the inhibitor bind to the enzyme?
A: Enzymes are usually protein molecules which catalyzes the biochemical reaction by decreasing the act...
Q: Starting with pyruvate (CH3COCOO—) as the precursor, the first step of gluconeogenesis is activatio...
A: Gluconeogenesis is a metabolic process that results in the transformation of non-carbohydrate carbo...
Q: What are all organic compounds made of?
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: what are the key glycolytic enzymes and why are they considered key? how are these steps overcome in...
A:
Q: 23. In the concerted model, the most active enzyme form will be when a. All subunits are in the R st...
A: Enzyme can have it's subunits in 2 conformations : Low-activity state -(Tense)T state High-activity...
Q: What amino acids are present on the unknown sample based on the results below? Test
A: Ninhydrin Test is used to test the presence of ammonia, amines or alpha amino acids or primary amine...
Q: Consider the following statements: (1) The term sugar is a general designation for both monosacchari...
A: Tge term SUGAR is a general designation for both mono and disaccharide. Glucose (monosaccharide) is ...
Q: Explain how protein is synthesized.
A: DNA undergoes transcription to synthesize mRNA. The mRNA synthesized through transcription is scanne...
Q: Which among the following indicates correct the three-letter abbreviation and one-letter abbreviatio...
A: There are twenty naturally occurring amino acids. These amino acids are conventionally represented b...
Q: 5. Treatment of the patients with familial hypercholesterolemia by statins (pravastatin, for instant...
A: Familial hypercholesterolemia is an autosomal dominant condition. It is linked to early cardiovascu...
Q: Is it ethical to see a severe need for help andnot respond to it directly?
A: Firstly, we need to understand what is a severe need . It may vary from person to person . The sever...
Q: 1. Amino acids are not stored in the body. Describe how excess amino acids are processed in the cell...
A: Hi. Thank you for the question, As per the honor code, We'll answer the first question since the exa...
Q: Oxytocin and vasopressin are peptide hormones which are also used as drugs. What are the functions o...
A: The growth of cells and body tissues, health depends on the presence of a sufficient quan...
Q: Basal Metabolic Rate (BMR) depend - on All Size of body O Mass of body O
A: “Since you have asked multiple question, we will solve the first question for you. If youwant any sp...
Q: 13. In an enzyme-substrate reaction, the reaction is favoured because 1elag ulomon3 A. The transitio...
A: Activation energy - Minimum threshold energy that needs to be provided for reaction to result in pro...
Q: One amino acid loses an oxygen and hydrogen atom from its and the other amino acid loses a a-carbon ...
A: Any of a group of organic compounds composed of a basic amino group, an acidic carboxyl group, and a...
Q: The charge of particular functional groups is dependent on the environment they are in. Predict the ...
A: The pKa of the side chains of amino acid residues in proteins determines the net charge on the amin...
Q: Consider the malate dehydrogenase reaction from the citric acid cycle. Given the listed concentratio...
A: Malate dehydrogenase is citric acid cycle enzyme (metabolism) catalyzes the oxidation of malate into...
Q: An enzyme is present at 100 nM (nanomolar) and has a Vmax value of 25 uM/s (micromolar/second). The ...
A: Enzymes are biocatalyst that increases the speed of reaction by lowering the act...
Q: How can a person make ethanol?
A: Introduction: It is commonly called alcohol, ethyl alcohol, and drinking alcohol. It is a neurotoxin...
Q: How is the beta-sheet different from the alpha-helix?
A: The structure of proteins have different levels of organization such as primary, secondary, tertiary...
Q: Look at the amino acid composition of a protein given in the table and click on the area in the 2D g...
A: the isoelectric point of an amino acid is the pH at which the net electric charge of that amino acid...
Q: Which precursors accumulate in patients with niacin deficiency. What effect does this deficiency hav...
A: Niacin is a precursor to nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleot...
Q: . Which type of proteins are also known as denatured proteins? A. Primary proteins B. Secondary pr...
A: Since you have asked multiple questions, we will solve the first question for you. If you want any s...
Q: Which of the following structural features are involved in the formation of a peptide bond? amino gr...
A: A polypeptide chain is a polymer of naturally occurring amino acids that are linked together by a pe...
Q: II. Give the test that will distinguish the ff. sugars. Indicate which sugar it is positive and what...
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature t...
Q: 1. At eukaryotic origins of replication, helicase cannot be activated until the polymerase is also p...
A: Introduction: DNA or deoxyribonucleic acid is a type of biomolecule known as nucleic acid. The impor...
Q: a sample that contains a carbohydrate delivers a negative starch iodine test. what does this result ...
A: Introduction: Carbohydrates are polyhydroxy aldehydes or polyhydroxy ketones with a molecular formu...
Q: What would be the pH level for an enzyme, that prefers acidic conditions.
A: The acidity or basicity of water is determined by its pH. The scale runs from 0 to 14, with 7 denoti...
Q: Modified true or false. Write the correct answer if the underlined word is false. 1. All standard a...
A: Any of a collection of organic compounds made up of a basic amino group (NH2), an acidic carboxyl gr...
Q: Click on the scheme on a functional group that has positive charge at pH 10.0: H2N. NH2 NH H2N COOH
A: The pKa of the ionizable groups of amino acids, play an important role in determining the net charge...
Q: Vitamin C is ascorbic acid. If you examine the structure of asorbic acid given in the background se...
A:
Q: II. The most highly sensitive test in viral hepatitis is y-Glutamyl transpeptidase (Y-GT) increased ...
A: Gamma-glutamyl transpeptidase is an enzyme present in the body. GGT test is the diagnostic test that...
Q: Amylopectin and cellulose are being compared. Which of the following choices explains the similarity...
A: Polysaccharides, also known as polycarbohydrates, are the most abundant carbohydrates found in food....
Q: Amount of water added (mL) Amount of Stock Solution (mL) Final Concentration A Solution 1 0. Solutio...
A: The concentration of unknown solution can be calculated from the formula C1V1= C2V2 Where C1= Concen...
Q: Why does L-amino acids prevail in biological systems?
A: Amino acids are organic molecules composed of carbon, nitrogen, hydrogen, and oxygen. Amino acids m...
Q: what are miscellaneous lipids?
A: Lipids get classified as - Simple lipid Compound lipid Derived lipid Miscellaneous lipid
Q: Which statement is NOT true of sterols? Question 8 options: Sterols have a structure that incl...
A: Sterols are fat molecules that are commonly found in cells. Sterols are non-polar molecules that are...
Q: Phytochemical test Physical description of extract: Color: Reddish brown Transparency: Turbid pH: Ac...
A: Antifungal agents are those that act against fungi and their growth. They might inhibit the growth o...
Ligand binding to proteins may occur with varying strengths; some ligands bind tightly to proteins while others bind less tightly. The strengths of reversible binding are determined experimentally by varying concentrations of ligands, and measuring the saturation of the protein in the various ligand concentrations.
One such laboratory study investigated the binding of a hormone to three different receptor proteins in the cell membrane. The data collected are shown in the table below
1) Which of the proteins demonstrate the tightest/strongest binding of the hormone? Which demonstrates the least
tight binding of the hormone? What is the basis for your answer?
Step by step
Solved in 2 steps
- BIM-46187 is a protein inhibitor that binds to the a-subunit of the G, protein. It prevents the GDP/GTP exchange and prevents activity of the G protein. Which of the following would you expect to see lower levels of as a result? You can select more than one answer. S-S Mol. W: 795.11 Image: https://aobious.com/aobious/protein-inhibitors/1086-bim-46187.html Select one or more: O a. Cyclic AMP (CAMP) O b. Tyrosine kinase HSP O d. Adenylyl Cyclase (AC) O e. DAG O f. JAK O g. IP3Starting from the situation below, explain the next immediate steps that occur after the agonist ligand binds to the GPCR. Describe what happens in terms of the GTP and the heterotrimeric G protein structure. www www Ligand α В GTP ZAN Y Copyright ©2009 Pearson Education, Inc. GDPserotonin [µM] 0.025 transport rate A [dpm/min] 300.0 transport rate B [dpm/min] 10 0.08 920.0 30.0 0.25 2500.0 100.0 0.7 5400.0 350.0 2 8800.0 1100.0 9100.0 3000.0 11. Draw a graph (by using Excel or similar), plotting the serotonin concentration against the transport rate under both conditions. 12. What transport rates for each condition would you expect if the experiment was repeated using 12µM ['H] serotonin? Add your estimate into the table above. 13. Examine the plot obtained for condition A; what can you conclude with regards to whether or not the transport of serotonin into these cells requires a protein? Explain your conclusion. 14. Compare the plots of the transport rates for both conditions. What is most likely mechanism of transport for serotonin into these cells? Explain your interpretation.
- serotonin [µM] 0.025 transport rate A [dpm/min] 300.0 transport rate B [dpm/min] 10 0.08 920.0 30.0 0.25 2500.0 100.0 0.7 5400.0 350.0 2 8800.0 1100.0 9100.0 3000.0 11. Draw a graph (by using Excel or similar), plotting the serotonin concentration against the transport rate under both conditions. 12. What transport rates for each condition would you expect if the experiment was repeated using 12µM ['H] serotonin? Add your estimate into the table above. 13. Examine the plot obtained for condition A; what can you conclude with regards to whether or not the transport of serotonin into these cells requires a protein? Explain your conclusion.Show where trypsin and chymotrypsin would cleave the following peptide. Tyr-Ile-Gln-Arg-Leu-Gly-Phe-Lys-Asn-Trp-Phe-Gly-Ala-Lys-Gly-Gln-GlnB. After treatment with peroxyformic acid, the peptide hormone vasopressin is partially hydrolyzed. The following fragments are recovered. Propose a primary structure for vasopressin.Phe-Gln-Asn Pro-Arg-Gly • NH2 Cys-Tyr-Phe Asn-Cys-Pro-Arg Tyr-Phe-Gln-AsnC. Consider the following peptide: Gly-Ile-Glu-Trp-Thr-Pro-Tyr-Gln-Phe-Arg-LysWhat amino acids and peptides are produced when the above peptide is treated with each of the following reagents?1. Carboxypeptidase2. Chymotrypsin3. Trypsin 4. DNFBD. From the analytical results, deduce the primary structure of a peptide isolated from the Atlantian orchid that contains 14 amino acids.Complete hydrolysis produces the following amino acids: Gly (3), Leu (3), Glu (2), Pro, Met, Lys (2), Thr, Phe. Treatment with carboxypeptidase releases glycine. Treatment with DNFB releases DNP- glycine. Treatment with a…The major enzyme that metabolizes caffeine is: о CYP1A2 0 CYP2B6 o CYP3A4 0 CYP2A6 о CYP2C9
- Despite the fact that many peptides have critical physiological functions(e.g., as hormones, neurotransmitters, antibacterial toxins), they are not considered to be ideal as drugs. The following questions illustrate some of the issues that limit the use of peptides as therapeutics.(a) Insulin is a peptide therapeutic used to manage Type 1 diabetes, whichaffects more than 20 million people worldwide according to the International Diabetes Federation. A significant limitation to the broad distribution and use of insulin to treat Type 1 diabetes is the fact that it must be administered by injection rather than orally. Why is insulin administered by injection and not orally?(b) Many of the D-amino acids found in nature have been discovered inbacterially produced peptides that have antibiotic properties. Bacteriasecrete these peptides into their environments to kill competitor bacteriaand thereby gain a selective advantage. Given your answer to part (a) ofthis question, what potential…Under normal physiological conditions, aspartic acid will be favored over aspartate. True FalseActivation of phosphatidylinositol-3-kinase (PI3K) stimulate protein-protein interactions. True or False
- The Graph below shows the binding curves of two proteins (A and B) for the same ligand (L). Use this Graph and determine the dissociation constant, K, for both proteins. Which protein (A or B) has a greater affinity for ligand L? Which of the two proteins would be more easily inhibited by an antagonist? 1.0 Y 0.5 2 A 4 6 B 8 [L] (μM) 10 12 14 16MAP= CO x TPR / 80 I don't understand the value of TPR how do I get this number?43. Sequentially list the signal transduction process from ligand binding to biological response for the phospholipase C system. You may use the appropriate abbreviations in your list.