Provide differences between solution-phase peptide synthesis and solid phase synthesis in terms of: 1.purification requirements 2.glassware requirements 3.solvent/reagent consumption 4.efficiency and automation 5. protection chemistry requirements 6. peptide coupling chemistry

Name some techniques/approaches (name, describe the major steps, how to analyze/interpret results)  that can be used to satisfy the following requirements:  1. Show that substance is pure protein 2. Only one protein was extracted

VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following significant information: Complete hydrolysis of the peptide yielded equimolar amounts of Leu, Orn, Phe, Pro, and Val Molecular weight of the peptide was estimated to be aroung 1,200 Da The peptide failed to undergo hydrolysis when treated with carboxypeptidase Partial hydrolysis and then chromatographic separation of products yielded the following di- and tripeptides: Leu-Phe, Phe-Pro, Orn-Leu, Val-Orn, Val-Orn-Leu, Phe-Pro-Val, Pro-Val-Orn Treament with dinitrofluorobenzene (DNFB) followed by complete hydrolysis yielded only free amino acids and the DNFB-derivatized ornithine at its side chain Using this set of information available to you, deduce the amino acid sequence of this peptide antibiotic. Write your final peptide sequence only using 3-letter abbreviations.

Give typed explanation