An attempt was made to inhibit Enzyme X by adding compounds M and L. The results are summarized in the table below: Which of the 2 compounds is the best inhibitor of Enzyme X? Show your computation. Assume equal concentrations of compound M and compound L. Km apparent, Vmax Type of Inhibition аpparent, umol/L mM/min Compound M 5.0 x 10-4 0.30 Non-competitive Compound L 2.5 x 10-2 0.40 Competitive Km Vmax Without inhibitor 5.0 x 10-4 0.40

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For a gradient system with a gradient of 5-90% in 50 min, flow 2 mL/min, column 100 x 4.6 mm i.d., the first peak elutes at 20 min, and the last peak elutes at 50 min. Calculate k* for this system.   2. Can isocratic elution be used for this sample? If so, what is the range of k values to be expected. 3.Propose a way to shorten the gradient run time by eliminating the wait for the first peak to elute at 20 min. 4. For the original conditions of this question, change the conditions necessary to use a 150 x 4.6 mm column while maintaining the same k* value.