. To which class does each enzyme belong? Explain your answers. a.) pyruvate decarboxylase b.) alanine aminotransferase c.) alcohol dehydrogenase d.) hexokinase

Discuss in a short paragraph (3–5 sentences for each mutation) whether or not the enzyme is likely to completely lose catalytic activity if the following mutations are carried out. You may assume that steric effects do not distort the active site so much that catalysis cannot take place; focus your analysis on discussing the chemistry the amino acid side chains are able to perform and the properties that enable them to do so.   Mutation 1: H to E Mutation 2: H to N Mutation 3: S to D Mutation 4: S to C

What is the name of the reactant, of the TCA cycle reaction, which yields a GTP? Selected Answer: succinate Correct Answer: Evaluation Method Exact Match Question 11 Bnous: Evaluation Method Exact Match Exact Match Exact Match onday, August 10, 2020 2:58:12 AM CDT Which enzyme of the TCA cycle catalyzes the reaction similar to the one that converts pyruvate to acetyl CoA? Selected Answer: dehydrogenase complex Correct Answer: Selected Answer: 2.5 Correct Answer: Evaluation Method Exact Match ✔ Exact Match Selected Answer: False Answers: Question 10 Question 2 How many moles of NADH will be produced when 1 mole of fructose-1,3-bisphosphate is metabolized to pyuvate and then through TCA cycle ✔True False Selected Answer: succinate Correct Answer: Question 9 The TCA cycle enzyme fumarase catalyzes a reversiable reaction. This enzyme shows a very high degree of selectivity in choosing the substrate for both the forward and the reverse reactions. Evaluation Method Exact Match Specified…

You have discovered a new enzyme that has a nearly identical active site to chymotrypsin. This new enzyme uses the same catalytic triad and the same reaction mechanism as chymotrypsin. Your new enzyme differs from chymotrypsin because it cuts peptides at the C terminus of polar, non- ionizable R groups. A) Beginning with the first tetrahedral intermediate, draw the mechanism of catalysis that occurs to cleave the tripeptide Asn- Phe- Lys substrate ending your answer with the product and free enzyme. B) From the list below, which of the components would most likely be found in the area of the enzyme that substitutes the hydrophobic pocket of chymotrypsin? Very briefly explain your choice(s): Ser ile Zn+ Val C) You've constructed a molecule that is able to bind to the 1st tetrahedral intermediate of your new enzyme, preventing catalysis. From experimental results, you can see that this molecule is only able to bind to the tetrahedral intermediate. Assuming that this enzyme follows…