Explain the purpose and overall technique for the following biotechnology applications: a. Isolation of Nucleic Acids b. Gel electrophoresis c. Polymerase Chain Reaction (PCR)

Q: Electrophoretic-Mobility Shift Assay DNA affinity chromatography Immunoprecipitation DNA footprint…

A: Introduction  DNA replication is the process by which a cell makes a copy of its genetic material,…

Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…

A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…

Q: Match the reagent with technique. Answer all for upvote Recombinant DNA technology a.)…

A: Recombinant DNA technology is a technology in which a set of techniques are used which enables the…

Q: Which of the following statement is an advantage of gene cloning in medicinal applications? I.…

A: Cloning is the creation of a replica that is perfectly similar. Copying short stretches of DNA…

Q: 1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: . List some of the beneficial applications of transgenicbacteria, plants, and animals.

A: Transgenesis is a technique of incorporating a gene or transgene in the genome of an organism taken…

Q: 4. Other reagents used in research labs are listed below. What is the meaning of the acronym and…

A: Enzymes are essential components of all metabolic processes in the body. They are catalytic…

Q: . The importance of recombinant DNA technology in pharmaceutical industry b. Potential products…

A: Recombinant DNA technology is an important tool as it allows for the manipulation of DNA  fragments…

Q: Which of the following sequence shows the correct order of the steps involved in Recombinant DNA…

A: Recombinant DNA (rDNA) technology is a set of techniques that allows the combination of DNA…

Q: A i. crossing over ii. biomarker a. Еcorl b. Recombinant iii. Gene-of-interest iv. restriction…

A: We know that crossing over is the major event during meiosis which leads to variations among…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes  Enzymes are the protein that use as catalysts in metabolic and catabolic reaction to fasten…

Q: Link the technique to the correct enzyme.…

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Q: Microarrays are used to monitora. the rate of DNA replication.b. the presence of particular genes in…

A: Introduction  Gene are the key component of genetic material which control almost all the cellular…

Q: 1. Write if the statement is true and modify the statement with the correct answer if its false…

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Q: One of the following can not be used in therapeutic cloning Select one: a. Embryonic b. Blood cells…

A: The cell is the smallest unit that can live on its own. The cell makes up all living organisms and…

Q: B. Genome Mining 1. Using the sequences from the previous activity, identify the organisms to which…

A: DNA sequencing - DNA sequencing refers to the general laboratory technique for determining the exact…

Q: main techniques used in biotechnology to genetically modify food

A: Genetic engineering Genetic engineering is an approach by which t genetic makeup of a cell is…

Q: Discuss the process and give example on the following: a. Genome Project-write b. Mitochondrial…

A: The process of genome writing, mitochondrial DNA "spring cleaning," micro-encapsulation, and…

Q: What is the difference between an antigen test and a PCR test?          a. What are the benefits…

A: PCR- PCR stands for polymerase chain reaction. Antigen test- Antigen tests are immuno-assays which…

Q: The CRISPR method is used in  a.DNA sequencing. b.DNA fingerprinting. c.genome editing. d.the…

A: CRISPER stands for clustered regularly interspaced short palindromic repeats. It is a group of DNA…

Q: Discuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell…

A: A. Aptamers are single-stranded nucleic acids that selectively bind to target molecules. Most…

Q: 1. What does PCR stand for? 2. Explain the main objective of the PCR technique.

A: PCR It is a technique used in molecular biology which was developed by Kary Mullis, an American…

Q: These functional groups act as acid/ base catalysts: A. His imidazole B. Thiol of Cys C. Guanidino…

A: Acid base catalysis is most common type of catalysis mechanism utilized by most of the enzymes like…

Q: 1. Which of these methods will allow cell counting and segregation? a. Flow Cytometry b. Western…

A: Introduction :- Bio-physical techniques or methods are combination of biological and physical…

Q: 1. After transformation, transfromants A. may possibly carry a non-recombinant plasmid B. refer only…

A: Since you have asked multipart question , we will solve only first three parts according to our…

Q: 1. Benedicts test is used to identify reducing sugars. What is the correct principle? A. Heating of…

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: X-ray Irradiation is   A. Chemical mutagen   B. Physical mutagen   C. Cosmetics agent   D.…

A: Mutagens are chemical compounds or forms of radiation (such as ultraviolet (UV) light or X-rays)…

Q: Give me 3 examples of how PCR and restriction enzymes can be used to create a genetic fingerprint

A: DNA is made of nucleotides, which are composed of a nitrogenous base, deoxysugar, and phosphate. The…

Q: Stems cells can be used in the following medical technologies. A. gene therapy  B. stem cell…

A: Stem cells are specialized cells present in various parts of the human body that are able to…

Q: Microarrays a. must be preceded by a sothern blot b. compare gene expression levels across many…

A: Here's why the other options are incorrect:a. must be preceded by a southern blot: Southern blots…

Q: You want to study a biomolecule in the laboratory. You have ordered the synthetic gene from a…

A: The process by which a specific gene sequence of interest is ligated and then the newly synthesized…

Q: Genetic information has become part of our culture and it is difficult to tell the difference…

A: Introduction :- Genetics is a branch of biology that studies genes, genetic diversity, and heredity…

Q: The Ames Test... a. is always used to detect cancer-causing virus b. has the potential to completely…

A: Ames test: A test to determine the mutagenic activity of chemicals by observing whether they cause…

Q: Cuts the DNA into fragments Circular DNA molecule of bacteria  Used to insert DNA of interest to…

A: Restriction enzyme - When this enzyme identifies its target sequence, it will form a double-stranded…

Q: 8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B.…

A: PCR amplifies a specific region of a DNA strand (the DNA target). Most PCR methods amplify DNA…

Q: indicate which of the following statements is/are FALSE for qPCR    A. The sequence order of steps…

A: qPCR Quantitative Polymerase Chain Reaction (qPCR) also known as Real-Time Polymerase Chain Reaction…