Explain the purpose and overall technique for the following biotechnology applications: a. Isolation of Nucleic Acids b. Gel electrophoresis c. Polymerase Chain Reaction (PCR)

Q: Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The…

A: Introduction DNA fingerprinting is a technique that is commonly used to identify people based on…

Q: 1.1 Give an overview of the positive and negative effects of microbial biotechnology in…

A: Microbial technology, also known as biotechnology or microbiotechnology, is the use of…

Q: Gel electrophoresis can be used for all of the following purposes except. (A) Determining the…

A: Gel electrophoresis is the technique of separating macromolecules like DNA, RNA and protein with the…

Q: Electrophoretic-Mobility Shift Assay DNA affinity chromatography Immunoprecipitation DNA footprint…

A: Introduction  DNA replication is the process by which a cell makes a copy of its genetic material,…

Q: Describe the role of restriction enzymes and gel electrophoresis in biotechnology.

A: Biotechnology is the field of biology used to solve many problems of the biological systems.…

Q: specific cell organelle for excretory ststem

A: The effective function of the excretory system hinges on the systematic removal of cellular waste…

Q: Match the reagent with technique. Answer all for upvote Recombinant DNA technology a.)…

A: Recombinant DNA technology is a technology in which a set of techniques are used which enables the…

Q: Which of the following statement is an advantage of gene cloning in medicinal applications? I.…

A: Cloning is the creation of a replica that is perfectly similar. Copying short stretches of DNA…

Q: Give at least four(4) uses/application of PCR. Explain.

A: PCR (Polymerase Chain Reaction) is use to amplify DNA. It can make billions of copies of a target…

Q: 5. a) Briefly explain three advantages that CRISPR has over earlier methods of genetic manipulation…

A: Introduction:- Genome editing is an extremely strong tool that can help you answer a wide range of…

Q: How can site-specific recombination be used in recombinant DNA technology

A: Recombinant DNA technology or rDNA technology deals with the manipulation of the genome of an…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: limitations of PCR viral diagnostic and explain how it could be improved

A: A significant biochemical technique that is used to generate multiple DNA (deoxyribonucleic acid)…

Q: Discuss three stages of TRANSFORMATION and RESTRICTION ANALYSIS OF PLASMID DNA experiment. What…

A: For transformation and restriction digestion analysis follow the given steps:  1. Cut the plasmid…

Q: Explain the Importance of protein separation in biotechnology

A: Introduction: The separation of proteins is an important step in biotechnology. It plays an…

Q: Which of the following statements about base editing is correct? 1. It involves nCas9. 2. The end…

A: Base editing  It is an alternative tool to HDR-mediated replacement. This facilitates editing of…

Q: Which of the following sequence shows the correct order of the steps involved in Recombinant DNA…

A: Recombinant DNA (rDNA) technology is a set of techniques that allows the combination of DNA…

Q: 1. Explanation of the benefits of bioinformatics in biological sciences. 2. explain the…

A: Bioinformatics is characterized as the use of tools of computation & investigation to the catch…

Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA

A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…

Q: Drawbacks of biotechnology in bullets point.

A: Biotechnology is the branch of biology that aims at developing products useful for mankind by using…

Q: A i. crossing over ii. biomarker a. Еcorl b. Recombinant iii. Gene-of-interest iv. restriction…

A: We know that crossing over is the major event during meiosis which leads to variations among…

Q: Determine how screening is carried out if pCR®II-TOPO® is used for cloning?

A: There are many applications of PCR as used in DNA fingerprinting to identify an individual from…

Q: 24. Arrange the correct sequence in southern blotting. I. DNA extracted from white cells and are…

A: The technique of transfer of gel to a membrane from the gel for the detection of sample on the…

Q: 8. Which of the following is/are TRUE about bioassay? 1. It is a dose-response type of research. II.…

A: As a result of comparing a substance's effect on a test organism with a standard preparation, a…

Q: 2. Answer the following questions (a) - (c) (a) What are the five necessary components to conduct a…

A: The PCR reaction exponentially increases the target DNA area by going through the annealing,…

Q: 1. Choose the phrase from the right column that best fitsthe term in the left column.a.…

A: The microbiology studies about both the diseases causing microbes and beneficiary microbes, about…

Q: One of the following can not be used in therapeutic cloning Select one: a. Embryonic b. Blood cells…

A: The cell is the smallest unit that can live on its own. The cell makes up all living organisms and…

Q: VII. Match the technique described to the application given. DNA amplification b. РCR dideoxy…

A: Introduction:-  Dialysis is a procedure which is used when kidney function is lost and excretion of…

Q: 3A.Below are a few (of the many) possible errors that could adversely affect PCR or analysis of PCR…

A: A PCR result can be disappointing in many ways. If DNA and primer concentration are reversed, the…

Q: 1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: What is the difference between an antigen test and a PCR test?          a. What are the benefits…

A: PCR- PCR stands for polymerase chain reaction. Antigen test- Antigen tests are immuno-assays which…

Q: Draw a diagram of what occurs in PCR during the annealing step of the FIRST cycle. b. Draw a diagram…

A: The PCR or polymerase chain reaction is a routinely used technique in molecular biology. It is used…

Q: 9) Describe the steps required to use sequencing DNA to identify your gene of interest after…

A: DNA sequencing is the process of finding the sequence of the nucleotides on a particular short…

Q: 2. PCR analysis practice One 1) If you collect samples from crime scene and extract DNA out of each…

A: For the amount of polymorphism in repeated sequences, any part of the DNA sample that is found in a…