Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Concept explainers

Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) a…
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6. Application: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no amplicon, or d) there are multiple, non-specific amplicons. Next to the possible cause, write which error is most likely associated with the cause (the first one is done as an example): Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon Annealing temperature too low hon specfic c amplication Poor primer specificity (wrong sequence) NO amplication of template sequence emurs in amplicahw ampl.caton in unngsize o n Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture amplicabon UN 米 Pick one cause and result pair and discuss in detail on the next page why one wouldy lead to the other:
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Transcribed Image Text:6. Application: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no amplicon, or d) there are multiple, non-specific amplicons. Next to the possible cause, write which error is most likely associated with the cause (the first one is done as an example): Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon Annealing temperature too low hon specfic c amplication Poor primer specificity (wrong sequence) NO amplication of template sequence emurs in amplicahw ampl.caton in unngsize o n Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture amplicabon UN 米 Pick one cause and result pair and discuss in detail on the next page why one wouldy lead to the other:
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