Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon hon specfic NO amplication of template sequence emors in amplicahon ampl cahoN in unngsize ampicahoN Annealing temperature too low amplication Poor primer specificity (wrong sequence) Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture Pick one cause and result pair and discuss in detail on the next page why one wouldy

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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6. Application:
There are four possible observations that suggest an error occurred in a PCR reaction:
a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no
amplicon, or d) there are multiple, non-specific amplicons.
Next to the possible cause, write which error is most likely associated with the cause (the
first one is done as an example):
Cause
Would result in the following error
Unbalanced nucleotide concentrations
a) Sequence errors in the amplicon
Annealing temperature too low
hon specfic
c amplication
Poor primer specificity (wrong sequence)
NO amplication of template
sequence emurs in amplicahw
ampl.caton in unngsize
o n
Template DNA degraded
Contamination of reaction tubes or solutions
Lack of Mg2+ in reaction mixture
amplicabon
UN
米
Pick one cause and result pair and discuss in detail on the next page why one wouldy
lead to the other:
Transcribed Image Text:6. Application: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence errors in the amplicon, b) the amplicon is the wrong size, c) there is no amplicon, or d) there are multiple, non-specific amplicons. Next to the possible cause, write which error is most likely associated with the cause (the first one is done as an example): Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in the amplicon Annealing temperature too low hon specfic c amplication Poor primer specificity (wrong sequence) NO amplication of template sequence emurs in amplicahw ampl.caton in unngsize o n Template DNA degraded Contamination of reaction tubes or solutions Lack of Mg2+ in reaction mixture amplicabon UN 米 Pick one cause and result pair and discuss in detail on the next page why one wouldy lead to the other:
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