1. Benedicts test is used to identify reducing sugars. What is the correct principle? A. Heating of simple carbohydrate is added with Copper (I1) forming copper (1) oxide which is an orange/ brick red complex. B. Polviodide ions is added to carbohydrate forming a black colored absorption complex, dextrin. C. Carbohydrate is added to H2504 undergoing dehydration and creating furfural which is combined with alpha paphtol creating a purple colored complex. D. None of the above.
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- VII. Match the technique described to the application given. DNA amplification b. РCR c. gel electrophoresis d. STR profiling dideoxy sequencing f. automated DNA sequencing g. site directed mutagenesis gene therapy h. 97. Nucleotides missing 3'-OH group makes small pieces that can be analyzed for identification. 98. DNA amplification without bacteria that includes cycling temperature changes to produce multiple copies of small pieces. 99. Identification of DNA by a series of peaks made from fluorescing based on fragment length. VIII. Match each item to the correct statement below. f. negative feedback system g. excretory system h. autonomic system i. positive feedback system j. parasympathetic nervous system homeostasis b. somatic system a. C. nervous system d. sympathetic nervous system endocrine system е. 100. way in which the body works to reverse a change and bring about normalcy 101. the tendency of the body to maintain a constant internal environment IX. Match each item to the…1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particles1. Write if the statement is true and modify the statement with the correct answer if its false a.White colonies represent those bacteria that do not express the genes for beta-galactosidase because restriction enzymes have cut the gene and ligase have not repaired the break b.Antibiotic resistance genes in bacterial transformation are used as marker genes because of ease of detection of transformants. c. Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell.
- These functional groups act as acid/ base catalysts: A. His imidazole B. Thiol of Cys C. Guanidino group of Arg D. All of the above Which of the following refers to discontinuous assay? A. also called sampling assay B. less prone to errors from sample manipulation C. assay is advantageous over other methods D. None of the above In cell cultures, ribozymes inhibit the expression of the following, except for: A. CMV- cytomegalovirus B. human HSV C. reovirus mRNA D. None of the abovefollowing site does the restriction enzymes act? 32. Restriction Fragment Length Polymorphism (RFLP) was used in order to identify the five different species of Staphylococci in a surgical ICU. Which of the following site does the restriction enzymes act? a. TAGATA/ATCTAT b. ATGGAC/TACGTG C. AATATA/TATAAT d. GATTAC/CATTAG theseWhat is the proper order of the following steps in a gene-cloningexperiment involving vectors?1. Add DNA ligase.2. Incubate the chromosomal DNA and the vector DNA with arestriction enzyme.3. Introduce the DNA into living cells.4. Mix the chromosomal DNA and vector DNA together.a. 1, 2, 3, 4b. 2, 3, 1, 4c. 2, 4, 1, 3d. 1, 2, 4, 3
- One of the following statements is FALSE A. Labeled antibodies are the tagged probes of Southern blotting and ELISA (enzyme linked immunosorbent assay) B. In biopharmaceutical engineering; immunogenicity and pharmacokinetic properties are altered favorably; hence therapeutically C. In RFLP (restriction fragment length polymorphism), number and length of restrictionfragments vary due to genome polymorphism and bases sequencevariations D. In gene replacement therapy a normal gene allele substitutes permanently a mutant allele in a somatic cell1. After transformation, transfromants A. may possibly carry a non-recombinant plasmid B. refer only to those carrying the recombinant DNA C. shall be wiped out after testing for biomarker 2. A polymerase chain reaction is facilitated by A. Recombinant DNA technology B. A thermal cycler C. Plasmid insertion D. DNA sequencing 3. Some plasmid sequences are available online. But we cannot select their promoters. A. True B. False 4. In gene reflection, which is part of a designated vector that cannot be part of the original plasmid? A. Foreign or transformed gene B. Ori site C. Multiple cloning site D. Resistance geneWhich of the following sequences in a bacterium CRISPR locus will vary and change O a. Spacer DNA sequences O b. Cas 1 gene O C. Cas 9 gene O d. Cas 2 gene e. CRISPR sequences CLEAR MY CHOICE The following techniques are considered in direct mutagenesis except O a. M13 enrichment protocol O b. M13 bacteriophage C. Using Degenerate Oligonucleotide O d. Using PCR Oe. Using plasmid CLEAR MY CHOICE What is the main trigger for the CRISPR system in bacteria O a. Bacteriophages envelop. O b. Bacteriophages Cas 9 enzyme CLEAR MY CHOICE
- 1. You have the plasmid pUC18/19, which is a circular plasmid that consists of 2686 bp. What would the number of and length of the fragments be if you cut the plasmid with the following restriction enzymes or combination of enzymes? Give a schematic representation of the digestions.a. PscI & GsuIb. ScaI, PdmI & BsaXI c.ScaI, SspI & EheIWhich of the following steps is NOT involved in gene cloning? Lütfen birini seçin: O a. Transferring recombinant vector into the host cell O b. Insertion of a gene into a vector O c. Selection of cells with recombinant vectors O d. Removing nucleus from donor cell O e. Isolation of a gene to be clonedExplain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.