These functional groups act as acid/ base catalysts: A. His imidazole B. Thiol of Cys C. Guanidino group of Arg D. All of the above Which of the following refers to discontinuous assay? A. also called sampling assay B. less prone to errors from sample manipulation C. assay is advantageous over other methods D. None of the above In cell cultures, ribozymes inhibit the expression of the following, except for: A. CMV- cytomegalovirus B. human HSV C. reovirus mRNA D. None of the above

Microarrays a. must be preceded by a sothern blot b. compare gene expression levels across many diffrent genes in two diffrent cell lines  c. require months to run e. are rarely used in modern biotecnology

8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B. Used to test for the presence of hundreds to thousands of active genes at the same tim C. Used as a vector to replicate foreign DNA inside bacteria D. Able to cut double stranded DNA at almost any desired location for genome editing Which of the following is not an example of RNA processing. the addition of the poly-A tail to mRNA in the nucleus spliceosomes cutting mRNA in the nucleus the addition of the 3' cap to mRNA in the nucleus the joining together of exons in the nucleus

Match description with Term:   1) determine the amount of protein in a sample 2) determine the location of a protein in a cell or sample 3) determine the amount of transcript in a sample 4) Determine the location of a transcript in a sample or cell   a. In situ hybridization b. northern blotting c. western blotting d. immunocytochemistry

Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdetermination

HindIII is a restriction endonuclease that recognizes a palindrome sequence. Predict which one is the HindIII recognition site: a. AAGGTT b. AAGCTT c. AGCCGA d. AGCGCA e. AGCAGC

Which of the following statements best describes the function of SDS in western blotting? Select one: A. It can solidify polyacrylamide gel. B. It allows the transfer of proteins from polyacrylamide gel to nitrocellulose membrane. C. It can form a colored product with proteins. D. It can linearize protein molecules. E. It gives proteins a net positive charge. O O O O

One would be correct in assuming that the restriction sites present in the multiple cloning site of the pUC18 plasmid will a. be the only instance of that restriction site in the plasmid b. appear at least once in another location in the vector. c. only include 4 bp restriction enzyme recognition sites. d. only be recognition sites that produce blunt ends when cleaved by the appropriate enzyme.

56. In gel electrophoresis, DNA is made visible with 5o. Use the following information to answer the next question. autoradiographic blue. a. Na - K pump situated in the neural membrane b. RFLP dye. C. agarose. d. polyacrylamide gel. Outside Inside ----K* diffusion e. ethidium bromide. Na carier 57. Gene therapy alters a patient's genome by Na* diffusion - adding bacteria to the genome. excising nucleotides. adding a corrected gene. engineering transgenic tissue. a. b. c. Which of the following correctly describes the ion flow into or out of a neural fibre during repolarization? d. e. bioremediation. K* flow out K* flow in a. b. 58. Use the following information to answer the next question. Na' flow out Na' flow in c. d. Neuron none of these e. Use the diagram given below to answer the next question. 61. Human Brain Myelinated neurons conduct impulses faster than unmyelinated neurons because in myelinated fibers, sodium gates open only a dendrite labeled 1 dendrite labeled 4 a. 2- b.…

Digestion of cellobiose in cows produces two glucose units which is then absorbed into the bloodand distributed into the cells for energy production. One glucose unit is catabolized in the muscle cellwhile the other is in the heart cell, to CO2 and H2O in the cellular respiration process.   How many ATP molecules are released? Show your detailed computation of the problem.