CAR) All une Red stock soluton concentration: Somple #tck AR/ vaume dilution 1 valume total AR Coantratin Abserbonce 50.00m4 50. 00mL 50.00 L So00 O. 16.4 O. 237 O. 392 10 dilution 3 20 dilution 4 25 312 unknarn - 0.485 Incude a printed copy of the colibration curve) Lolibrotion curve cayotion: R valve
Q: Change in Weight Based on Concentration of Solution % Weight Change= Change F-I)/I X Solution…
A: A line graph is used to study the relationship between two different variables.
Q: Amoxicillin/clavulanate potassium (AUGMENTIN) powder for oral suspension is prepared prior to…
A: Amoxicillin-clavulanate potassium for oral suspension is generally used as an antibacterial and…
Q: Protein concentration standards 0.6 0.5 0.4 0.3 0.2 0.1 0.5 1 1.5 2.5 Albumin concentration (mg/mL)…
A: Proteins are essential macromolecules/polymers that are composed of peptide bound amino acids. They…
Q: Discuss how the ratio of unfrozen water at different temperatures affects the food's stability in…
A: The ratio between the vapor pressure of the food itself, when in full equilibrium with the…
Q: In a neutral solution, the [H] is : O 1.0x10-14M zero 1.0×107M 1.0x10-7M
A: The acidity of any solution is determined by the molarity of the Hydrogen ions. So, the pH of any…
Q: ceftazidime
A: Accurate dosing is very important in the field of healthcare. Overdosing and underdosing both are…
Q: concentration? The maximal rates A 30 → B· →C- (Enter the numerical value only)
A: The following metabolic pathway is provided to determine the rate of formation of the end product E
Q: To determine the metabolizable energy (ME) of a petfood, I can use Modified Atwater Values (3.5, 3.5…
A: Metabolizable energy calculations is determined by modified Atwater equation.
Q: aelis-Menten constant an be determined using the Lineweaver-Burk plot and is equal to twice the…
A: Mechaelis-Menten Constant: - Km is used in the enzyme kinetics, it plays very important role in the…
Q: Ib 20 n moles OL produch are obsenved In 10 minutes and 50 nmoles of obsen ved is V. 2 product in 20…
A: The reaction rate or rate of reaction or Vo is the speed at which a chemical reaction takes place,…
Q: Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of…
A: Introduction Molecular biology deals with various techniques for study of structure and functioning…
Q: epare 100 cfu/mL bacterial suspension. Present in diagram if necessary and show all calculations…
A: A colony-forming unit, or CFU, is a measurement of microbe concentration in a test sample. To get a…
Q: Calculate the volume of BSA stock that will be required to make the standard solutions needed to…
A: A stock solution is a concentrated solution that is diluted to make a working solution. It is used…
Q: DATA TABLE L6108.3 nm Lamda max Trial Concentration (mol/L) Absorbance 0.428 0. 8०० 1.17 1.498 1.845…
A: Spectroscopy is the interaction between electromagnetic radiation and matter. If transmittance T…
Q: Boctors order Tazicef (ceftazidime) 1.5 g IV Q12H. Refer to the label below. How much Tazicef will…
A: Order dose Tazicef (ceftazidime) 1.5 g IV q 12h
Q: Compute the cfu/g (lactic acid bacteria and coliforms) of burong mustasa samples given the following…
A: The total coliform bacteria test is the simplest basic test for bacterial contamination of a water…
Q: A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing…
A: IV or Intravenous fluid when injected into patients has to be carefully regulated as it would…
Q: a. Order: Oxacillin 500 mg IV q. 6hSupply: Oxacillin 2 g, multi-dose vialDirections: Reconstitute…
A: 30 mL 4 doses 1 Day
Q: At 10 p.m. , a patient must receive 1g of Cefazolin (Ancef) intravenous piggyback (1.V.P.B.) in…
A: At 10 p.m. , a patient must receive 1g of Cefazolin (Ancef) intravenous piggyback (1.V.P.B.) in…
Q: Calculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.
A: Introduction Agarose gel electrophoresis is a type of gel electrophoresis used in biochemistry,…
Q: PLEASE ANSWER THEM ALL FOR AN UPVOTE. CHOOSE TRUE OR FALSE. AND JUSTIFY IT.
A: In this questions asked about the preparing drug from the available solution.
Q: You are asked to make 10 mL of buffer from a 5X concentrate. invitrogen Te rerder visit…
A: In their working range, buffers resist change in pH. Buffers consist of a weak acid and its…
Q: Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B…
A: The medication employed for curing fever, inflammation is known as aspirin and it is a chemical…
Q: a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product…
A: Given values in the question are as follows: mAb = 120 mgl Total volume of the solution = 5 l…
Q: 14 strength Pulmocare 450mL q8h followed by 100mL of water after each feed. Calculate the mixture,…
A: Given, that 1/4 strength pulmocare 450 mL q8h has to be given followed by 100 mL water after every…
Q: Ndel 183 Aatil 2617 Sspl 2501 Ehel 235 Pdml 2204 Bogl 2215 Scal 217 146/ 230 MCS lacz 2486 PUC18…
A: INTRODUCTION Screening techniques It inclued Blue white assay, Agarose gel electrophoresis, replica…
Q: (i) (iv) 3.0 2.5 2.0 1.5 1.0 0.5 0.3 (ii) (iii) Air 20°C 101525Pa Limin
A: Bioreactor: It consist of a vessel where biological reactions takes place through the transformation…
Q: 7. Bromophenol Blue, a reagent or standard that is usually used to determine the void volume of a…
A: NOTE: since you have posted multiple questions, So we will be solving the first three parts for you.…
Q: dissolue 5 gm Qs) Q9) Compute (wt/wH)% Solution prepured by Ans.: y. 76% Sodium chloride in loo ml…
A: Given that 5gm of sodium chloride is dissolved in 100 ml water . And we need to find the…
Q: Which of the following indicates buffering in the titration given in the image below? 12 1000000 11…
A: A buffer is a aqueous solution which is used to resist the pH change upon addition of acid or base.…
Q: Derive a general expression for the minimum filter area requirement per a sterility assurance limit…
A: The concept of sterility defines a matter completely devoid of microorganisms that are viable. This…
Q: Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______…
A: Dilution is the technique of lowering the concentration of a solute in a solution by merely adding…
Q: Amo Velocity [Vo] in umoles/min/ml of [s] in milligrams [S] in % OD at 540nm maltose 1/Vo 1/[S] in…
A: Michaelis-Menton plot is drawn using Michaelis-Menton equation to determine Km and Vmax of the…
Q: Five identical biood samples were analyzed to deterttmine cholesterol level in a patient The…
A: Mean is the term used to tell the average of the given set of numbers or values. This is also called…
Q: Indinavir has a pKa of 5.0, and 10 (A) what is the % dissociation in stomach (pH =8)
A: The nurses administer the drugs to the patient, and thus it is very much necessary for the nurses…
Q: Substrate Concentration (mol L-1) Velocity (mM min-1) 2.500 0.588 1.000 0.500 0.714 0.417 0.526…
A: Lineweaver-Burk plot also known as the double reciprocal plot is used to graphically represent the…
Q: Compute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values…
A: Violet Red Bile Agar- is the medium where the coliforms bacteria grow develop sufficiently. The…
Q: value range for Ca/O ratio in succinate? a)3.2-3.5 b)4.0-4.3 c)4.4-4.7 d)3.6-3.9 e)none
A: The above question is related to Oxidative phosphorylation. Oxidative phosphorylation is metabolic…
Q: Calculate the of the acetic acid in for each – show all unit conversions (hint: start with mL of and…
A: The balanced chemical equation of vinegar with NaOH is as given below: CH3COOH + NaOH →CH3COONa +…
Q: 1. compute for patient B result of his chloride analysis with the following data using mg/dL unit…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: a. The feed to the precipitation unit has 120 mg mAb/l and 28 mg impurity/l in 5 liters of product…
A: Given values in the question are as follows: mAb = 120 mgl Total volume of the solution = 5 l…
Q: Compute the cfu/g (lactic acid bacteria and coliforms) of burong mustasa samples given the following…
A: Introduction The colony forming unit (CFU) is a measurement indicating the number of viable…
Q: Show calculations for making 10 mls each of the following dilutions of sucrose from the 0.30 M stock…
A: Dilution is defined as a term that refers to the solution that is made by adding solvent to a…
Q: H druclose IS 2.5mM Gnd &0mM From letl→ to right monine@ 25°c what is the eneryy change? inK/mol
A: During a chemical reaction, in order for the reaction to take place, a few or all chemical…
Q: The absorbance values for different BSA standards. How can the protein concentration be calculated?…
A: The Bradford protein assay is used to measure the total protein in a given solution. It is a…
Q: components of robert's reagent
A: Robert's reagent is type of chemical reagent which is used for determining the quality of albumin…
Q: A patient is prescribed Cipro for an upper espiratory tract infection. The oral suspension is…
A: The concentration of cipro in the oral suspension is 200mg/5ml (milligram per milliliter) which can…
Q: Michaelis Menton Plot and a Lineweaver-Burke plot using this data
A: Michaelis-Menten plot describes the increase in rate of an enzyme catalyzed reaction with respect to…
Step by step
Solved in 6 steps with 1 images
- NAZO NHZ Ala-Cys-Glu -Tyr - Trp - Lys - Arg - His -Pro-G ly Glu pka 4.15 SH Tyr 10.10 Draw Charges Lys 10.67 Olt A3 12.10 +NH₂ Ntrm 2) Calculate net charge 3) write out I letter code 300 Ctim 3 juli of peptich (above) Ⓒ pH; 1,7,12Cells in the human liver contain glycogen, a polysaccharide that is used as a source of energy. This is a model of a typical glycogen granule: 42 22 AWARD Edit View Insert Format Tools Table be *** www 27544 Hals De w Jay MASCH Abh P CON p bo H S ******** 124 3. P WH ** patink The G k page flees P ******* 44651 CAPE Porn Tele de to MATE www 74 H Supe This type of granule may contain up to 30,000 (3 x 104) glucose subunits, making up the glycogen chains. A. How many ATP's could theoretically be produced when a granule of glycogen is hydrolyzed to glucose subunits that then enter glycolysis and respiration? B. Glycogen metabolism is regulated by insulin and glucagon. Both of these hormones are produced by the pancreas and act as signals that interact with a transmembrane receptor on target cells. Intracellular events involve protein kinase A that activates or inactivates enzymes involved in glycogen synthesis or degradation. Briefly describe what protein kinase A does to these proteins.O Ara (Alt+ Ğ) Yeni. - Gözden Geçir Görünüm Yardım Başvurular Posta Gönderileri 1) Discuss this graph in the meaning of substrate depletion. 100 80 [S] [P] 60 40 20 20 40 60 80 100 Time (s.) ere goz atın 7SUS 17 19 5 8. 9. Relative Concentration
- During the recovery stage of annealing, the grain size [Select] v and dislocations [ Select) Materials with higher melting points tend to have [Select] recrystallization temperatures. As we increase the annealing temperature for a recrystallized material, the grain size generally is I Select ] fiet blmk cheices! stays the same, decreases, inccesses is not predicfable Secand blak.choices: (Carrarge toreduce energs disappear, iameese in density third blank choices: higher, aweg unpredictable farth blank choices: lacger Smeller unpredic fableFigure 3: ● ● ● ● ● ● KDa ● 97.4 66.2 45.0 ● 31.0- 21.5 14.4 S-1 p-1 S-2 2-0 This figure was generated by centrifuging a pura sample of protein, removing the supernatant, and resuspending the pellet in the same volume as the supernatant to allow direct comparison. The supernatant and pellet samples were then prepared for SDS-PAGE identically and run via normal SDS-PAGE procedures. In the figure, "s" means supernatant and "p" means pellet. The text or number after the dash represents a different condition. For example, s-1 and p-1 are the supernatant and pellet samples under condition 1. It is not shown, but under wild-type conditions, essentially all of the protein is found in the supernatant. S-3 ● What does the intensity of each band represent? ● Would you find soluble protein in the supernatant or pellet? Why? Would you find aggregated protein in the supernatant or pellet? Why? For each condition (there are 5 different conditions), is there a higher percentage of the total protein…The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?
- Vnohes sdTfend old Srlaieol word otsuixorggs animsasb ne wng o2 11 If0.1 ml of a 10-3 dilution of a culture is plated out and 65 colonies appear after incubation, how many CFU/ml were in the undiluted culture?1. Lactoferrin is an iron-binding glycoprotein that forms the antibiotic fragment lactoferricin during digestion. The protein was isolated from 1.5 liters of ewe skim milk by successive ammonium sulfate precipitation until a final salt concentration of 70% was obtained. Determine the amount (in g) of powdered ammonium sulfate needed in bringing the solution from (a) 0% to 40% saturation and (b) 40% to 70% saturation assuming that the volume remains constant. Initial concentration of (NH4)2SO4, % saturation 0 10 20 30 40 50 60 70 80 90 10 55 Final concentration of (NH4)2SO4, % saturation 30 40 50 60 70 80 Grams of solid (NH4)2SO4 to be added to 1 L of solution 176 242 314 390 472 561 657 117 405 491 584 59 421 351 438 20 113 57 182 121 61 251 325 63 188 126 195 130 65 260 337 270 202 281 135 67 210 140 70 90 761 685 511 609 533 365 292 219 146 HUGH LATLE 100 73 457 381 305 228 152 76Calculate your dilution strategyto make a 625 ng/mLHRP solution from a 12.5 μg/mL stock given the totalvolume of enzyme youwillneed for your condition this week. Note if you are testing pH, you only need to calculate one enzyme dilution because all other pHs will use the same strategy.
- 1:55 .ll 0.52 KB/S 56 1.pdf CHEM 3141: BioChemistry Quiz no. 1- Answer key I.) Draw the following compounds. 1.) Maltose: 2 Glucose, a-(1,4) 2.) Gentobiose: 2 Glucose, B-(1,6) 3.) C-2 epimer of Talose 4.) C-3 epimer of Fructose 5.) C-2 epimer of ribose II. Predict the products of the following reactions, if there is none, write NO RXN. Also indicate, if the reaction is fast or slow он FO HO -H H -OH но. + Resorcinol/dilute HCI 1.) 2.) Sucrose + Copper (II) Acetate/Acetic Acid HO H HO H HO H H OH 3.) HO + Orcinol/HCI1.2. Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Cys, His and Leu Starting pH: _____ Ending pH: _____1. Calculate the approximate MW of a protein with 341 amino acid residues and express it ing/mol and in kD. Show complete solution.