Composition of Cell Lysis Buffer Volume or Weight of Reagents Needed Required Concentration of Reagent Molecular Weight 40 mM Tris HCI 20 mM Acetic acid 157.59 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 5% SDS ddH20 95.21 288.37 18.02

Q: Describe the full procedure/protocol you would follow in order to determine the activity of the…

A: Unpleasant environmental factors such as drought stress lead to elevated levels of harmful ROS in…

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: The following set of data are measurements of zones of inhibition (in mm) from a Kirby-Bauer test.…

A: The Kirby-Bauer test, also known as the disc diffusion test, is a standard method used to determine…

Q: Give typing answer with explanation and conclusion    why do we use the multitest system known as…

A: Enteropluri test contains a special culture medium for the identification of bacteria. It is a…

Q: Interpret Benedict's reagent results by filling in the table below. To fill out the table, choose…

A: Carbohydrates are the compounds which are polyhydroxy aldehydes or polyhydroxy ketones or their…

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: As a safety measure, serum cholinesterase is measured before the examination takes place. Explain…

A: The piece of information is necessary for determining the correct dosage of succinylcholine to…

Q: Question) In the factory where you work, we can provide you with hydrolysis of lactose, glucose and…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: Show the de novo synthesis of capric acid. Please help me with this illustration thank you

A:

Q: When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient…

A: HPLC is expanded as High Performance Liquid Chromatography. It helps in excluding out the analyte in…

Q: Give the principle involved in the ninhydrin test. What is its purpose?

A: The principle involved in the ninhydrin test is that the two molecules of ninhydrin react with the…

Q: What is the interpretation and reaction catalyzed of this GPT enzyme test?.

A: The GPT test, also known as the Alanine Aminotransferase (ALT) test, is a blood test used to measure…

Q: Search for the principles and the uses of the following methods for protein measurements and provide…

A: In the question 5 different methods of protein measurement are given. Serum Protein Electrophoresis…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: 4. How would you summarize the effect of enzyme concentration on enzyme activity?

A: Enzymes are biological catalysts that increase the rate of reactions taking place in the living…

Q: An iodine solution was sealed inside dialysis tubing, then immersed in a cup of water containing…

A: Dialysis membrane is a semipermeable membrane and allows only a few substances to pass on as…

Q: True or false Explain your answer To observe the Bacterial species E. coli with bright field…

A: Bright field microscopy is a widely used technique in which specimens are observed under normal or…

Q: A cyclic AMP (CAMP) binding protein was isolated from mammalian cells and characterized by…

A: Given that,The ligand is cAMPThe receptor is cAMP binding protein (CBP)also the concentration of CBP…

Q: A continuious, stirred-tank bioreactor with an actively growing culture of bacteria is operating at…

A: A bioreactor is a manufactured device or a system which supports biologically active environment…

Q: Can anyone assist in providing a detailed description of each step of the following purification…

A: Pectinase are group of enzymes which catalyzes the degradation of pectic substances. It occurs…

Q: 3) Define gel electrophoresis, including its theory and application. Describe the steps of running…

A: Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Q: 6. Why did the enzyme activity differ in the ice bath compared to the boiling water bath?

A: Enzymes are mostly proteins in nature hence their activity gets affected by the factors such as…

Q: the 10 mg/mL sample of lysozyme shows a lower activity than 2mg/mL lysozyme sample (refer to the…

A: The most likely explanation for the lower activity of the 10 mg/mL lysozyme sample compared to the 2…

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: You are planning an experiment to treat cells in culture with compound A at a final concentration of…

A: In an experiment, a control group is a group that doesn't get the treatment being tested. Its…

Q: What do the following abbreviations mean: DdDp, RdDp, DdRp, RdRp. Which could be provided by cells?

A: Answer: Introduction: RNA-dependent RNA polymerase (RdRp) is also called as RNA replicase, it is a…

Q: Writé án appropriate aim for the students' experiment. p) What is the purpose of beaker 2 in this…

A: Dialysis : It is a procedure to remove waste products and excess fluid from the blood when the…

Q: A scientist tries to harvest bacterial cells by centrifugation; he used an RPM of 5000. If the…

A: Centrifugation is a process in which molecules are segregated out at high speed but as different…

Q: What is the role of a coagulating agent in the process of purification? (please be direct to the…

A: Treatment of waste water is done by different types of chemical and physical methods in which…

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: When viewed under a microscope, bacteria appear as red rods after they have been stained. Explain a…

A: Introduction Bacteria Are Minute, Single-celled Organisms That Can Be Found In Large Numbers In All…

Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…

A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…

Q: Consider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean…

A: Batch culturing of microorganisms is an important part of industrial applications of microbiology.…

Q: determine the concentration of glucose in an unknown solution. Mention how you will calibrate the…

A: Spectrometer is a type of instrument, which is used to measure the transmission of UV or visible…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: Absorbance is directly proportional to glucose concentration for both routine clinical glucose…

A: Quantitative tests help to determine the concentration of target substance in the solution. The…

Q: Explain how the Kirby-Bauer test works and what information it provides. Define minimum inhibitory…

A: Bacteria are the primary cause of the majority of infectious diseases. It has become vital to…

Q: You perform a succinate dehydrogenase (SDH) assay on the fractions the isolated fractions. You read…

A: An absorbance value above 2 gives inaccurate approximation. Hence, if we get an absorbance above 2,…

Q: You perform the Methyl-Red(MR)& Voges-Proskauer(VP) test and gather the following results:…

A: MR-VP Broth is recommended for use in the performance of the Voges-Proskauer and Methyl Red tests as…

Q: The energy required for diffusion of a solute across a cell membrane is: the kinetic energy of the…

A: As according to our guidelines, we are allowed to do a single question at a time, if you want second…

Q: The Bradford reagent gives a linear response only from 0.1 mg/mL to 1.4 mg/mL of protein…

A: There are several biochemical tests that are used to determine the concentration of protein in a…