1.2. Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Cys, His and Leu Starting pH: _____ Ending pH: _____

54. A patient with a viral infection is receiving 200 mg of didanosine PO bid. The pharmacy only has powder packets with 167 mg each, to be mixed with 4 oz of water. How many ounces should the patient take per dose? _________ ounces. Round to the tenth place.

For 4-aminosalycilic acid please pick only the second choice phase 2 metabolite per handle present in the drug: OH H,N 4-Aminosalicylic acid OH إختر واحدة أو أكث OH OH NH, HN HO. OH HN HN он HO HO он HQ HO. HO. HN

A purified protein fraction has a total sample volume of 360 µL.  The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052.  (Both values were measured with a path length of 1.00 cm.)  If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).