Calculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.

How much agarose need for 40 mls of 1.5%w/v gel

When comparing the structures of acetate buffer and tris-maleate buffer, acetate buffer works on fumarase activity but tris-maleate buffer does not?

The purification continues with a cation exchange step in which the positively charged cytochrome C protein is separated from negatively charged DNA and other proteins. The cation exchange eluate (volume of solution collected) had a total volume of 42.0 mL and a 1.0 mL aliquot was set aside for further analysis. The following data was obtained from the 1.0 mL aliquot to quantify the protein amount and purity: The absorbance at 410 nm of the aliquot was diluted 5-fold was 0.474 (1 cm pathlength). The absorbance at 595 nm from a 1.0 mL Bradford Assay solution that was diluted by 100-fold from the aliquot was 0.195 (1 cm pathlength). Using the information given, Calculate the total protein amount in mg from the absorbance at 595 nm. Calculate the cytochrome C amount in mg from the absorbance at 410 nm using Beer’s Law.

Explain the Rf value obtained on the basis of the structure of amino acids use. Explain in 1-3 sentence only.

Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock solution.