Calculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.

Q: Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and…

A: Introduction: Lipids are defined as esters of alcohol and fatty acids which are water-insoluble and…

Q: How can a mass spectrometer be used to identify the protein/s excised from a 2D gel?

A: Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio…

Q: On an SDS-gel, If the distance traveled by the bromophenol blue dye is 7 cm, and the distance…

A: Given values:  Distance traveled by the dye front = 7 cm Distance traveled by the protein = 2.8 cm

Q: Compute the pI value of tyrosine. If the gel in an electrophoresis experiment is at pH 7, in which…

A: Step 1 Gel electrophoresis (Tiselius,1937) is a technique of separating biomolecules under an…

Q: You have prepared a BSA serial dilution (ug ml) to construct a protein standard curve, for which the…

A: The standard equation for calculating concentration is Beer Lambert's law, A= ebc, where A is…

Q: Choose among proteins A, B, C, and D. 1. Protein that will most strongly bind to an anion exchange…

A: Chromatographic techniques are used to separate specific molecules from a mixture of different…

Q: Nonspecific elution of affinity bonded macromolecules is used in affinity chromatography explain…

A: Chromatography techniques are often used in biochemical research to purify proteins and other…

Q: When eluting your analyte of interest using HPLC, gradient elution can be used. How does Gradient…

A: HPLC is expanded as High Performance Liquid Chromatography. It helps in excluding out the analyte in…

Q: Three dipeptides are separated by electrophoresis at ph 5.8. if the dipeptides are Arg-trp, Asp-thr,…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: Agarose gels can vary from 0.8 to 2% explain why we use different gel percentages

A: Gel is a type of matrix which is used in the electrophoresis method to separate the organic…

Q: A mixture of proteins contains Trypsinogen (24 kDa), fumarase (49 kDa), Transferrin (80 kDa) and…

A: Chromatography is a form of an analytical method that breaks down a chemical mixture into its…

Q: if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992…

A: Answer:- The standard curve gives the absorption value of aliquots having the substance of known…

Q: 4. An enzyme catalyzed reaction was initiated in 1.0 mL of 0.06 M HEPES buffer, pH 7.55. During the…

A: The Henderson Hasselbalch equation describes the mathematical relationship between the pH and pKa of…

Q: The order is for Moxam 5 gm IM every 8 hours.  On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: Why is agarose an ideal gel matrix for electrophoresis experiments? Because it forms a liquid at all…

A: Nucleic acids are negatively charged molecules because of the phosphate backbone. Due to their…

Q: Show the order of elution from a cation-exchange resin using a buffer pH of 4.5: aspartic acid,…

A: A cation exchange resin is one that exchanges cations. The resin in this is negatively charged. At a…

Q: A large chaperone protein complex GroEL is approximately 16 nm in diameter. When it is dissolved in…

A: Particles present in cells show rapid movement(back and forth) and we call it Brownian motion. The…

Q: In nickel column affinity chromatography，how does the protein binds to column？How is the protein…

A: Column chromatography is a very common method of protein purification.Proteins may vary hugely in…

Q: 24.22 As part of a bioseparations process, glucose (solute A) in aqueous solution (solvent B) is…

A: Diffusion is defined as the process of movement of molecules along the concentration gradient i.e…

Q: From this standard curve and chart below, does the separation of molecules in the mixture appear…

A: Size exclusion chromatography is a technique used to separate molecules by the virtue of their…

Q: How to prepare %25 100 ml Glucose solution?

A: Dextrose solution, also known as intravenous sugar solution, is a combination of dextrose (glucose)…

Q: If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the…

A: The biuret assay is a colorimetric method used to quantify the concentration of proteins in a…

Q: Diffusion experiments of a small molecule drug in tissue samples are performed. The drug has a…

A: The ability of a dug to diffuse through the cell membrane is called diffusivity. Lipophilic drugs…

Q: Given this: Protein Isoelectric pH Molecular weight (kDa) Ovalbumin 4.6 45 Insulin 5.4 5.7…

A: The primary technique for proteomics research is two-dimensional gel electrophoresis. It uses two…

Q: suctose density gradient ultractifugation is a powerful technique for fractionating macromolecules…

A: The sucrose density gradient is used for fractionating the DNA, RNA, and protein. The samples are…

Q: How would you make up 200ml of blue sepharose CL-6B stored in 0.1M sodium phosphate buffer pH 7.0,…

A: Sepharose is primarily cross-linked agarose beads commercially available. It is commonly used to…

Q: Give two benefits of utilizing the biuret reaction to assess protein concentration over directly…

A: A Biuret test is a generic test performed by molecules having two or more peptide links (CO-NH…

Q: hypothetical

A: Isolation and characterization of plant and human pathogenic bacteria from green pepper (Capsicum…

Q: what type of gel must be used when the protein size is 2500 Da

A: A type of size exclusion chromatography in which the separation of components is based on the…

Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

A: DNA concentration of a sample can be determined using spectrophotometry and measuring the absorbance…

Q: Why is necessary to completely grind the beef in an LDH purification experiment?  How do you…

A: Since you have posted multiple questions, we will provide the solutiononly to the first question as…

Q: A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish…

A: The largest organ of the body, the skin, is composed of water, protein, lipids, and minerals. It…

Q: briefly explain the physical mechanism by which amino acids are separated during paper…

A: Paper chromatography is a type of plane chromatography where the stationary phase is present on a…

Q: A 40 umol.l-1 solution of flavin mononucleotide (FMN) gave an absorbance of 0.8 at 470 nm using a 1…

A: Beer law states that the concentration of the solution is proportional to the amount of light it…

Q: Hand draw the expected gel result from the graphical data of three extracted RNA samples (in Figure…

A: Nanodrop spectrophotometer is a technique which helps in measuring the nucleic acid that is DNA/ RNA…

Q: Make an analysis of the electrophoresis data below. Comment on the purity, number of subunits, and…

A: Native gel electrophoresis is the process of separation without adding any denaturing agent like…

Q: In RP HPLC, one of the most common separation methods used to measure purity, strength, dosage, etc,…

A: High Performance Liquid Chromatography (HPLC) is biochemical separation method for organic…

Q: Use correct sig figs The concentration of a purified monoclonal antibody was measured using UV280…

A: Monoclonal antibodies are laboratory-produced molecules that are engineered to mimic the immune…

Q: Write the biochemical structures of bicarbonate buffer  Phosphate buffer and protein buffer.…

A: Bicarbonate buffer system It is used to balance pH in blood and duodenum along with some other…