oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive

1. Here is a DNA sequence . Give the complimentary bases. ATT  CGG  TCG  CGC  TTA  ACG

13. Southern blotting is separation of DNA by agarose electrophoresis and probing with a labeled primer. By analogy, Eastern blotting is: A a method that analyzes nucleotides and small RNA fragments B. a method that analyzes ribosomal RNA C. a method that analyzes transfer RNA D. a method that analyzes messenger RNA E. all of the above F none of the above

HO a. b. d. 8. Consider the following strand of mRNA. a. What would the original template DNA have been? b. What protein sequence would result from this strand of MRNA End End a. DNA MRNA 5' AUG – CCG - UAG - UGC - CGA – UAG b. Protein 9. In the DNA double helix, why are purine bases on one DNA strand never paired with purine bases on the opposite strand? There is more than one reason. 31 C.

Hind III' Hind III .9 kb .9 kb Bam HI plasmid A .9 kb .7kb Bam HI .3 kb plasmid B .9 kb .4 kb .3 kb .5 kb/ Eco RI Bgl Il .4 kb Eco RI .7 kb .5 kb -Bgl II Bam HI Eco RI You requested that a lab run by a friend of yours share a couple of plasmids that they created with you. She was willing to send you the ones that you asked for in exchange for citation in any papers that were published using the plasmids. Eco RI However, the labels from two vials have fallen off in the shipping because the adhesive used to hold them was not able to withstand the temperature, being shipped on dry ice. Each vial contained a different plasmid and now you do not know which plasmid is in which. Fortunately, you have restriction maps for both plasmids, shown above. Explain how you would determine which vial contained each plasmid and give a graphical representation of your expectations related to your method of Bam HI discovery. (25pts)

which letter dipicts leading strand A. J B. B C. K D. L

13.Which lane or lanes on the gel could be the result of Mutation C?

The nucleic acid of a virus isa. DNA onlyb. RNA onlyc. both DNA and RNAd. either DNA or RNA

2. A. B. C. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. G = % % A = % In the bacteriophage DX174, the DNA is approximately 26% A. C = % G = % A = T= Sea urchin DNA is approximately 18% G. C = % T = % T= % % 64