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oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive

oth DNA (D) and RNA a. What is the KM b. What is the KM C. What is the Vm d. What is the Vm e. On the graph, c noncompetitiv f. On the graph, uncompetitive

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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2200 cleave 2000 1800 I 1/V R 1600 • 1/V D 1400 1200 1000 800 600 400 200 rly. -1 0 1 5 6. 8. 10 11 y. 1/S (uM)-1 ts resulted in a kcat of 79 min1 and a KM of 87 µM. urk plots (using kcat for the M-M plot and Vmax for letely. 1/V (uM x min-1)-1
Transcribed Image Text:2200 cleave 2000 1800 I 1/V R 1600 • 1/V D 1400 1200 1000 800 600 400 200 rly. -1 0 1 5 6. 8. 10 11 y. 1/S (uM)-1 ts resulted in a kcat of 79 min1 and a KM of 87 µM. urk plots (using kcat for the M-M plot and Vmax for letely. 1/V (uM x min-1)-1
1/V (uM x min-1)-1 1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave both DNA (D) and RNA (R). 2200 2000 1800 a. What is the KM when cleaving DNA? I 1/V R 1600 • 1/V D b. What is the KM when cleaving RNA? 1400 1200 C. What is the Vmax when cleaving DNA? Z 1000 800 d. What is the Vmax when cleaving RNA? 600 e. On the graph, draw an appropriate line for a 400 noncompetitive inhibitor for cleaving DNA. Label clearly. f. On the graph, draw an appropriate line for an uncompetitive inhibitor for cleaving RNA. Label clearly. 200 8 10 11 1/S (µM)-1
Transcribed Image Text:1/V (uM x min-1)-1 1. To the right is a Lineweaver-Burk plot for an enzyme that can cleave both DNA (D) and RNA (R). 2200 2000 1800 a. What is the KM when cleaving DNA? I 1/V R 1600 • 1/V D b. What is the KM when cleaving RNA? 1400 1200 C. What is the Vmax when cleaving DNA? Z 1000 800 d. What is the Vmax when cleaving RNA? 600 e. On the graph, draw an appropriate line for a 400 noncompetitive inhibitor for cleaving DNA. Label clearly. f. On the graph, draw an appropriate line for an uncompetitive inhibitor for cleaving RNA. Label clearly. 200 8 10 11 1/S (µM)-1
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