
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
The TPOX locus is one of the genetic loci used for
What type of molecular marker is TPOX?
a copy number variant
a simple tandem repeat
a single nucleotide polymorphism
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Forensics DNA profiling can be used to solve old or unsolved crimes and to identify the person responsible for the crime. The availability of parental DNA samples can also allow identification of a body when conventional means are unsuccessful. These markers can also be used to establish the identity of missing persons. A dissimilarity in DNA profiles can be used to exclude a relationship.
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- Select all that apply: A SNP (single nucleotide polymorphism) is similar to an STR (short tandem repeat) in that: a) an individual can have many different copies of specific SNP or STR b) they both cause diseases c)they are sequences that have many possible variants d) an individual has one copy of this region of the genome from each parent.arrow_forwardIn this western blot, the levels of actin increase with increasing amounts/expression of the viral protein (VP). Figure description: Increasing amounts of a plasmid expressing the viral protein (0.5, 1, or 2ug) were cotransfected with TBK1 expression plasmid. Cells were harvested 24 h post-transfection and analyzed for phosphorylated TBK1 (anti-PTBK1 Ser172), total TBK1 (anti-TBK1), B-actin (anti-ß-actin), and viral protein (anti-VP) expression by Western blot analysis. VP PTBK1 (S172) TBK1 actin Virus proteins O True O Falsearrow_forwardPlace the steps of the double-strand break model of recombination in order. DNA polymerase fills in gaps in the DNA exonuclease generates 3' single-stranded overhangs DNA ligase seals the nicked DNA First step Last step Answer Bank homologous region of intact homolog is invaded by free end endonuclease cleaves both strands of one homologarrow_forward
- Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…arrow_forwardIf the researchers carry out a PCR for this gene from the CD8+ T cells, what result would you expect?Explain.arrow_forward#2.) Mary wants to further confirm if she is a carrier for this lethal gene. She was told that a new SNP marker was identified RFPL marker. She requested to do SNP test for all the DNA samples. The results are: her parents are heterozygous for SNPs (that is to say, one chromosome is wild-type and another one contains the SNP). The sister who passed away is homozygous for the SNP. Mary does not have this SNP. So now what is the probability of Mary as a carrier of the death gene?arrow_forward
- The following sequence contains the first couple codons from the Moderna vaccine mRNA. Below it are versions of the same sequence, but each of these contain a mutation. Match these mutated sequences with the correct mutation type a b C d Original sequence AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC UAG UGC GUG AAC CUG AUG UUC GUG UCC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCA CUG GTG AGC AGC CAG UGC GUG AAC CUG AUG UUC GUG UUC CUG GUG CUG CUG CCC CUG GTG AGC AGC CAG UGC AGU GAA CCU 1. Synonymous 2. Nonsense 3. Non synonymous 4, Frameshiftarrow_forwardwhen various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forward
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