You work in an environmental microbiology research lab, and your project is to design a recombinant bacterial strain that will be beneficial to the environment in some way. You decide to add recombinant DNA to E. coli. The DNA encodes an enzyme capable of oil catabolism. The resulting recombinant E. coli strain is able to metabolize and degrade oil from marine environments. O Genotypic Change Only O Phenotypic Change Only O Both Genotypic and Phenotypic Changes O Neither Genotypic nor Phenotypic Change

You work in an environmental microbiology research lab, and your project is to design a recombinant bacterial strain that will be beneficial to the environment in some way. You decide to add recombinant DNA to E. coli. The DNA encodes an enzyme capable of oil catabolism. The resulting recombinant E. coli strain is able to metabolize and degrade oil from marine environments. O Genotypic Change Only O Phenotypic Change Only O Both Genotypic and Phenotypic Changes O Neither Genotypic nor Phenotypic Change

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.
5 strains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, and only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and colonies are observed the next day. Rich Media (Lb) E5 E4 E1 E2Ⓡ E3 MM + Mal+Leu E5 E4 X ● E1 E5 X MM + Mal E4 X X E1 E3 E2 E5 ✪✪ E2 E4 ● E1 E5 MM + Mal+Leu +Pro E2 E3 MM + Ara E4 X X E1 E3 E2 X X Colonies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 - E5)
Which of the following apply to the development of urogenital tract Candida albicans infections? Disruption of population dynamics allowing the RB form of Chlamydia to cause tissue damage Loss of Lactobacillus populations leading to an increase in the local pH Loss of Gardnerella vaginalis populations leading to a lose of Clue cells Loss of the anaerobic environment that promotes the outgrowth of Trichomonas vaginalis None of the answers apply
. In an interrupted-conjugation experiment in E. coli, thepro gene enters after the thi gene. A pro+ thi+ Hfr iscrossed with a pro- thi- F- strain, and exconjugants areplated on medium containing thiamine but no proline. Atotal of 360 colonies are observed, and they are isolatedand cultured on fully supplemented medium. These cultures are then tested for their ability to grow on mediumcontaining no proline or thiamine (minimal medium),and 320 of the cultures are found to be able to grow butthe remainder cannot.a. Deduce the genotypes of the two types of cultures.b. Draw the crossover events required to produce thesegenotypes.c. Calculate the distance between the pro and thi genesin recombination units.
In a genetics lab, Kim and Maria infected a samplefrom an E. coli culture with a particular virulent bacteriophage. They noticed that most of the cells werelysed, but a few survived. The survival rate in theirsample was about 1 × 10−4. Kim was sure the bacteriophage induced the resistance in the cells, whileMaria thought that resistant mutants probably alreadyexisted in the sample of cells they used. Earlier, for adifferent experiment, they had spread a dilute suspension of E. coli onto solid medium in a large petri dish,and, after seeing that about 105colonies were growingup, they had replica-plated that plate onto three otherplates. Kim and Maria decide to use these plates totest their theories. They pipette a suspension of thebacteriophage onto each of the three replica plates.What should they see if Kim is right? What shouldthey see if Maria is right?
You discover a new bacteria and are interested in mapping its genome with respect to 10 genes. You are able to infer time-of-entry maps for four Hfr strains, which are pictured below. What is the map of the bacterial genome based on these Hfr experiments? Hfr1 Ga E Ne Hfr2 Sq Lix Tf Ne Hfr3 U I D Ga Hfr4 D U Sq O Sq - Lix - Tf - Ne -E- M- Ga - D -1-U O Sq - Tf - Lix -U -E-D- Ne -D -M- Ga OD-1-U- Sq - Lix - Ga - E -M- Tf - Ne OD- Ne - Ga -E- M - Sq - I-U- Tf - Lix O Lix - Ga - E - Sq -1-U- M -D- Tf - Ne
E. coli cells are simultaneously infected with two strains of phage λ. One strain has a mutant host range, is temperature sensitive, and produces clear plaques (genotype h st c); another strain carries the wildtype alleles (genotype h+ st+ c+). Progeny phages are collected from the lysed cells and are plated on bacteria. The following numbers of different progeny phages are obtained: Progeny phage genotype Number of plaques h+ c+ st+ 321 h c st 338 h+ c st 26 h c+ st+ 30 h+ c st+ 106 h c+ st 110 h+ c+ st 5 h c st+ 6 a. Determine the order of the three genes on the phage chromosome. b. Determine the map distances between the genes. c. Determine the coefficient of coincidence and the interference
Categorize the following as being associated with transformation, conjugation, or transduction. Note that some items may be associated with more than one process. Transformation Conjugation Transduction requires a virus can transfer dead bacterium's DNA to a new host cell efficient way to get naked plasmid into E. coli cells in the lab involves a pilus describes type of horizontal gene transfer in bacteria cells need to be competent process exploitod by biotechnology transfer requires at least two living bacteria alters bacterial genome
Most yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture. These are called flor yeast. The following Southern blot is looking at the FLO11 gene that is thought to be involved in whether yeast are flor or not. DNA was isolated from four different yeast strains. Strains 1 and 3 are normal while strains 2 and 4 are flor (Fidalgo, 2006). Based on the gene map and Southern results below, what aspect of FLO11 determines whether a strain will be flor or not?
You are trying to isolate a mutant of wild-type E. coli thatrequires histidine for growth. This can best be done using1. direct selection.2. replica plating.3. penicillin enrichment.4. a procedure for isolating conditional mutants.5. reversion.a) 1, 2 b) 2, 3 c) 3, 4 d) 4, 5 e) 1, 5
You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
Which of the following statement about conjugation is TRUE: During F+ to F- mating, the donor becomes F-. During the Hfr to F- transfer, the recipient becomes F+. The whole bacteria genome may get transferred during Hfr mating. During the Hfr to F- transfer, some recipients become recombinants in which transferred fragment recombines with homologous region in the genome.