Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is55,290 mol "dm cm, calculate the concentration of the molecule that produced anabsorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working-13-1

Given Values: ε=55290 c = ?A = 0.77 l = 1.5 cm

Answered: Assuming the molar extinction…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Assuming the molar extinction coefficient for para-nitrophenyl phosphate (PNP) is 55,290 mol "dm cm¯, calculate the concentration of the molecule that produced an absorbance of 0.770 OD units in a 1.5 cm cuvette. Show all working -1 3 -1