1. If 100 mls of 0.05 M NaoH is added to 700 mls of 0.15 M glycine buffer at pH 9.4, what is the resultant pH (PK1 for Gly= 2.2, Pk2=9.4) glycine .7LX.15mole/l = .105 moles NaOH .1LX.05mole/L = .005 moles Glyº .0525 moles Start Add.005 moles NaOH -.005 End .0475 moles .059M Gly + H+ .0525 moles +.005 .0575moles .071M pH = pka +log [A-]/[HA] =9.4 + log .071/.059 = 9.5

b) Among the progeny from the Hfr3 mating above, you find one that has the genotype: Abc de F Draw out the gene transfer and crossover(s) that produced this outcome: c) Among the progeny from the Hfr3 mating above, you find one that has the genotype: A B c d e f Draw out the gene transfer and crossover(s) that produced this outcome:

Would you be able to draw a circular map of the seven genes? I have trouble with correct position of the genes

What is the Lineweaver-Burk plot and what does it tell us?