Although several different mammalian species have been cloned, the efficiency of this process is extremely low. Often tens or even hundreds of oocytes must be implanted with donor nuclei to obtain one healthy live birth. Many researchers believe the difficulties with cloning reside in the epigenetic modifications, such as DNA and histone methylation, that occur within various cells during an individual’s life. How do you suspect such modifications might affect the success of an experiment

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: What color is the alpha gene spot on the microarray? Answer: Yellow please explain why is the…

A: The microarray can be described as a lab-on-a-chip. It can be used to detect the expression of…

Q: Research has now demonstrated that techniques that have been developed for cloning animals could be…

A: Introduction: Therapeutic cloning is also referred to as somatic cell nuclear transfer or SCNT. It…

Q: Telomerase is not active in most human cells. Some cancer biologists have suggested that activation…

A: Telomere is a repetitive nucleotide sequence added at each end of the chromosome. It acts as a…

Q: Describe the methodology that you would use to determine the association between specific sequence…

A: Determining the affiliation between particular hereditary variations and heritable hereditary…

Q: If you were to design an epigenetic experiment, how would you design one? Keep in mind a good…

A: Epigenetics is the gene-level alterations that affect the functioning of DNA without actually…

Q: A wild type strain of Drosophila was found to carry a transposable element called copia. From this…

A: The following is a short summary of the genetics terminology and Drosophila notation used in this…

Q: The goal of most gene therapies is to insert a healthy copy of a gene into the genome.  Besides…

A: Genome editing:It is also known as gene editing or genome engineering. It is a type of genetic…

Q: Could quantitative PCR, which uses a DNA-binding dye, to show how many copies of the target DNA…

A: Quantitative reverse transcription PCR (RT-qPCR) is used when the starting material is RNA. In this…

Q: When a particular mutagen identified by the Ames testis injected into mice, it causes the appearance…

A: Cancer is considered to be a disease that has abnormal cell growth that can invasive. The cancerous…

Q: Cloning of a gene from the rubber tree can be carried out in bacterial cells. However, for the…

A: Cloning: It refers to the process of creating multiple identical copies of gene-sized pieces of DNA.…

Q: om college and started working at a biotech startup called Scrofa first job assignment is to clone…

A: Prolactin is defined as a hormone by the pituitary gland at the bottom of the brain. Prolactin…

Q: In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they…

A: In the setting of assessing treatment for a modern patient (patient B) who may develop comparable…

Q: Unneeded genes in an adult animal cell are permanently inactivated,making it impossible for most…

A: The genes are the hereditary unit of an organism which are passed on from the parental generation to…

Q: In yeast, you have sequenced a piece of wild-type DNA and it clearly contains a gene, but you do not…

A: To find the mutated genes one must code all the amino acid of the genetic modified yeast and normal…

Q: In this module, you have learned about the use of balancer chromosomes (such as TM2 and TM6C in the…

A: Lethal genes are alleles that result in an end of the entity that possesses them. In basic terms,…

Q: Not long ago investigators were surprised to learn that more than 95% of a mammalian genome is…

A: Introduction  There are around 25000 genes present in the human genome which have around 3 billion…

Q: You want to clone a eukaryotic gene and express the protein in yeast. The protein typically…

A: Cells are divided into organelles which differ in their protein composition and cells must target…

Q: Gerald Rubin and Allan Spradling devised a method of introducinga transposon into Drosophila. This…

A: Plasmids are the genetic structures, which can replicate independent of chromosomes. They are the…

Q: Studies on monkeys have provided insight on the role of maternal care on the epigenome. Imagine you…

A: The study to examine the role of epigenetics in shaping animal / human behaviour is called as…

Q: You just graduated from college and started working at a biotech startup called Scrofabulous. Your…

A: Gene is cloned with the help of polymerase chain reaction(PCR). PCR is a rapid in vitro method to…

Q: Bacteria are often the preferred hosts for splicing in novel genes. For instance, the gene for human…

A: "Biotechnology" is the use of our knowledge of biological processes for the development of…

Q: What is genetic engineering? Describe one method genetic engineering can be done (use your book or…

A: Genetics can be defined as the branch of biology which deals with genes, their variation, hereditary…

Q: Imagine you are working in a lab that is developing a novelty rose that smells like a stinkhorn…

A: Introduction :- Recombination DNA technology is used for making clones of genes ( gene cloning) ,…

Q: From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is…

A: Transgenic approach has been proved to be very useful in studying physiological function of a…

Q: In a qPCR experiment, you find that the mRNA for the gene CDK1 goes down (decreased expression) when…

A: qPCR (quantitative polymerase chain reaction), also known as real-time PCR, is a laboratory…

Q: A researcher studying epigenetic changes induced by exercise had participants pedal a stationary…

A: Introduction DNA is the molecular structure that is composed of a pair of polynucleotide chains and…

Q: Cloning of a eukaryotic gene can be carried out in Escherichia coli. However, for the protein…

A: Introduction Eukaryotes are organisms having well-defined cellular organelles that are specified to…

Q: As we described in class, in the early 1960's Francis Crick and colleagues set out to determine how…

A: Introduction  Genetic code   The sequence of bases that encodes a functional protein molecule is…

Q: A certain compound that is an analog of the base cytosinecan become incorporated into DNA. It…

A: The genome is made up of one to several long DNA molecules, and mutations on these molecules can…

Q: Expression of recombinant proteins in yeast is an important tool for biotechnology companies that…

A: Recombinant proteins are those proteins that are produced by recombinant DNA cloned in a system…

Q: Treatment of adenosine deaminase (ADA) deficiency is an exampleof ex vivo gene therapy. Why is this…

A: Gene therapy is a collection of methods that allows correction of a gene defect that has been…

Q: Because genetic engineering involves the modification of an organism's set of instructions, it has…

A: * Genetic engineering means it is the process of using rDNA technology to alter the genes of…

Q: What is the relationship between methylation and genomic imprinting? O Differential methylation of…

A: The DNA methylation is the process by which the gene expression is turned off. That means the…

Q: By whole-exome sequencing, you have identified an early termination mutation in KLHL4 in a human…

A: "Hi, since you have posted more than one question, we will be answering the first two for you.…

Q: Woolly mammoths have been extinct for about 4,000 years, but we often find their well-preserved…

A: Somatic cell nuclear transfer (SCNT) is a technique in which the viable embryo is developed from egg…

Q: The restriction digests of the cloned Drosophila gene can provide direct visible evidence of a…

A: We can use the technique of Southern blotting to detect and isolate the DNA fragment with the Gene…

Q: Geneticists have been exploring ways to suppress the expression of the extra chromosome 21 in…

A: Chromosomes are a compact form of DNA wrapped around some proteins and are generally present in a…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.