From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment. a. How can you redesign the knockout to get past embryonic lethality? b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?
From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment.
a. How can you redesign the knockout to get past embryonic lethality?
b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?
Transgenic approach has been proved to be very useful in studying physiological function of a gene, pathophysiology of a disease caused by genetic disorder like cancer. To determine the function of a gene of interest, it is often over-expressed by insertion of a transgene or knocking-out for reduction in its activity.
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