From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment. a. How can you redesign the knockout to get past embryonic lethality? b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy
Section: Chapter Questions
Problem 12QP: In selecting target cells to receive a transferred gene in gene therapy, what factors do you think...
icon
Related questions
Question

From gene expression studies on cancer, you discovered a hypothetical gene called ABCG2 that is overexpressed in human cancer cells. You want to know if this gene is required for cancer cells to survive, but from your efforts at making a traditional gene knock out, you have found that it is required for embryonic development and can't get to the point of doing a cancer experiment.

a. How can you redesign the knockout to get past embryonic lethality?

b. How would you then use this mouse line to test if ABCG2 is needed for cancer survival?

Expert Solution
Step 1

   Transgenic approach has been proved to be very useful in studying physiological function of a gene, pathophysiology of a disease caused by genetic disorder like cancer. To determine the function of a gene of interest, it is often over-expressed by insertion of a transgene or knocking-out for reduction in its activity.

trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 1 images

Blurred answer
Knowledge Booster
Mutation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Heredity: Principles and Issues (MindTap Co…
Human Heredity: Principles and Issues (MindTap Co…
Biology
ISBN:
9781305251052
Author:
Michael Cummings
Publisher:
Cengage Learning