In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they have reasons to believe might develop a cancer similar to another patient they treated successfully (patient A). They know that the severity of the cancer is mainly associated with mutations in a specific gene (BRCAI). Suggest a technique that can be used to rapidly assess the similarity between the genetic pakerns of patients A and B, without the need to sequence the entire gene, and briefly describe it. A team of scientists are interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (0) The sequence the scientists are interested in is 5'-CATTGATTATTGE...JATCAATTACGGG-3' 3-GTAACTAATAAG[...]TAGTTAATGCCC-5* Where [...] indicates a longer 100bps sequence. Provide two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid. Briefly explain the reason behind your choice. Explain how the team can verify that their selection of primers was able to restrict the implementation to only that region of the plasmid.
In a clinical context, doctors are evaluating a therapy for a new patient (say patient B) that they have reasons to believe might develop a cancer similar to another patient they treated successfully (patient A). They know that the severity of the cancer is mainly associated with mutations in a specific gene (BRCAI). Suggest a technique that can be used to rapidly assess the similarity between the genetic pakerns of patients A and B, without the need to sequence the entire gene, and briefly describe it. A team of scientists are interested in the amplification of a specific DNA fragment in a large plasmid of about 10000 bps. (0) The sequence the scientists are interested in is 5'-CATTGATTATTGE...JATCAATTACGGG-3' 3-GTAACTAATAAG[...]TAGTTAATGCCC-5* Where [...] indicates a longer 100bps sequence. Provide two possible primers that the scientists should use to address their need, if they want to be sure they specifically address this region in the entire plasmid. Briefly explain the reason behind your choice. Explain how the team can verify that their selection of primers was able to restrict the implementation to only that region of the plasmid.
Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
![In a clinical context, doctors are evaluating a therapy for a new patient (say patient B)
that they have reasons to believe might develop a cancer similar to another patient
they treated successfully (patient A). They know that the severity of the cancer is
mainly associated with mutations in a specific gene (BRCAI). Suggest a technique
that can be used to rapidly assess the similarity between the genetic panerns of patients
A and B, without the need to sequence the entire gene, and briefly describe it.
A team of scientists are interested in the amplification of a specific DNA fragment in
a large plasmid of about 10000 bps.
(0)
(11)
The sequence the scientists are interested in is
5'-CATTGATTATTG[...JATCAATTACGGG-3"
3-GTAACTAATAAG[...]TAGTTAATGCCC-5*
Where [...] indicates a longer 100bps sequence.
Provide two possible primers that the scientists should use to address their
need, if they want to be sure they specifically address this region in the entire
plasmid. Briefly explain the reason behind your choice.
Explain how the team can verify that their selection of primers was able to
restrict the implementation to only that region of the plasmid.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc8545c89-19c2-4d90-bc33-b5c0e87e8b3c%2Fa8711d2f-0b81-413b-828b-c856069e94cc%2Ff7tg827_processed.jpeg&w=3840&q=75)
Transcribed Image Text:In a clinical context, doctors are evaluating a therapy for a new patient (say patient B)
that they have reasons to believe might develop a cancer similar to another patient
they treated successfully (patient A). They know that the severity of the cancer is
mainly associated with mutations in a specific gene (BRCAI). Suggest a technique
that can be used to rapidly assess the similarity between the genetic panerns of patients
A and B, without the need to sequence the entire gene, and briefly describe it.
A team of scientists are interested in the amplification of a specific DNA fragment in
a large plasmid of about 10000 bps.
(0)
(11)
The sequence the scientists are interested in is
5'-CATTGATTATTG[...JATCAATTACGGG-3"
3-GTAACTAATAAG[...]TAGTTAATGCCC-5*
Where [...] indicates a longer 100bps sequence.
Provide two possible primers that the scientists should use to address their
need, if they want to be sure they specifically address this region in the entire
plasmid. Briefly explain the reason behind your choice.
Explain how the team can verify that their selection of primers was able to
restrict the implementation to only that region of the plasmid.
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