In yeast, you have sequenced a piece of wild-type DNA and it clearly contains a gene, but you do not know what gene it is. Therefore, to investigate further, you would like to find out its mutant phenotype. How would you use the cloned wild-type gene to do so? Show your experimental steps clearly

You want to clone a eukaryotic gene and express the corresponding protein in yeast. However, the protein typically localizes within mitochondria. How will you perform your gene cloning so that the protein is secreted from the cell, rather than localized within yeast mitochondria?

ou are studying a new gene “X” that you think controls skin color in Bearded Dragons. In order to determine what gene X does, you need lots of gene X DNA to work with. So, you decide to amplify it through the process known as PCR. You have a forward and a reverse primer that have been designed to gene Xf – a gene in frogs that is similar to the one found in the Bearded Dragon. You are pretty sure that these two primers will work in Bearded Dragons, so you decide to go ahead with the PCR. In frogs, the Forward Xf primer binds gene Xf between nucleotides 32 and 47. The Reverse Xf primer binds between nucleotides 1110 and 1135.   a. What size PCR product would these two primers create if they amplified the frog gene Xf??

You obtained the sequence of the frog gene X you amplified in Question #16 through a process called automated sequencing. In automated sequencing, you are given a printout of the sense strand of your DNA.  The first thing you need to do is use the correct reading frame. Having done this, the next thing to do is to write out the mRNA sequence using this sense strand reading frame. The last thing to do is to translate the sequence.  a.The reading frame DNA sequence is: b.The mRNA sequence is: c.The polypeptide sequence is: A disease in frogs which causes their tongue to fall out of their mouths is killing the frog population in LA County. You obtain a dead frog and isolate its gene Xf. When you sequence this mutated gene, you find that the last ‘G’ at the end of the first line of this sequence has been deleted (i.e. the G at position 86). In order to determine how this mutation changes the resulting polypeptide, write the mutated polypeptide sequence in the space below. What kind of…

Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.

In the experiment described above, state what each of these parts of the experiment was:   Independent variable Dependent variable Control group Experimental group One controlled variable