om college and started working at a biotech startup called Scrofa first job assignment is to clone the pig gene for the hormone prolactin. Assume that th for prolactin has not yet been isolated, sequenced, or mapped; What would be the mo economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to to PCR-amnlifu
om college and started working at a biotech startup called Scrofa first job assignment is to clone the pig gene for the hormone prolactin. Assume that th for prolactin has not yet been isolated, sequenced, or mapped; What would be the mo economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to to PCR-amnlifu
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:You just graduated from college and started working at a biotech startup called Scrofabulous. Your
first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene
for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and
economical first step to go about identifying and cloning the pig gene for prolactin?
O use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers
to PCR-amplify the pig prolactin gene.
RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin
Conduct a proteome search for peptides that match parts of mouse prolactín protein
Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin
O Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid
sequence in the pig proteome
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