All of the following statements are correct EXCEPT a. DNA forms described by Watson and Crick have right handed helix except of Z-DNA form b. In case of Z-DNA form, the deoxyribose phosphate backbone forms a "Zigzag structure" c. Inverted DNA sequence repeat that happens within each individual strand of the DNA, called cruciform d. Plasmid carries genes that convey antibiotic resistance to the host bacterium

Which of the strands of DNA could act as a PCR primer for the DNA sequence shown below? 5’-CCCTGGGCTCTGTAAATGTTTCTAAGTG-3’ 3’-GGGACCCGAGACATTTACAAAGATTCAC-5’   A. 3′-ACTGTTAGA-5′   B. 3′-AAATTTGGC-5′   C. 3′-ATGCTTTGA-5′   D. 5′-GGGACCCGA-3′   E. 5′-CCCTGGGCT-3′

In the Watson-Crick structure of DNA, the: a. adenine content of one strand must equal the thymine content of the same strand. b. nucleotides are arranged in the A-form. c. purine content (fraction of bases that are purines) must be the same in both strands. d. two strands are parallel. e. the strands are complementary to each other.

Which of the following is NOT TRUE about replication? A. Polynucleotides are made up of deoxynucleotide monophosphates but the substrates are deoxynucleotide triphosphates B. The mechanism of the reaction is nucleophilic addition C. In the formation of primers, nucleotides are added in any direction D. Both nucleotide triphosphates and deoxynucleotide triphosphates are substrates in replication E. Direction of synthesis is 5'23' and lengthens unidirectionally relative to the double strand.

Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…

The image below was the basis of Watson and Crick to be able to elucidate the DNA structure. Explain/Discuss the meaning of this image as seen by Watson and Crick and how was it able to support the present-day structure of the Watson and Crick DNA?

A small section of Saccharomyces cerevisiae gene has the amino acid sequence valine, histidine, cysteine, and lysine. A mutation in the above section of the amino acid sequence resulted in the substitution of amino acid histidine with amino acid glutamine. The mutation in the antisense strand DNA of Saccharomyces cerevisiae gene described above involves a. the substitution of thymine base from GTG b. the deletion of second cytosine base from CAC c. the deletion of second guanine base from GTG d. the substitution of second cytosine base from CAC

A mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of the mutant DNA strand? The sequence of the mutant strand is shown below: 5'-TGCCATAACTGTTCGTACTGGCAAATTGCC-3' 3'-ACGGTATTGACAAGCATGACCGTTTAACGG-5' b. The mutation altered the sequence of the wild type template DNA such that a degenerate codon for a basic amino acid in the wild type was converted to a non-degenerate codon resulting in the sequence for the mutant strand shown. What was the original amino acid? c. Compare the charges and pl of the mutant peptide and the normal (wild- type) peptide at physiological pH?

PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?