Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Question
The following is a section of DNA removed from a cell nucleus:
5' ATGAAATAATCAGTTAACAGCAGVFCCGATTTTTATACT 3'strand
3' TACITTATTAGTCAAVFGTCGTCAAGGCTAAAAATATGA 5'strand
a. What does the Central Dogma state?
b. Label the strands above as the "sense" or "antisense" strand.
c. Using the chart below, transcribe ONLY the gene into mRNA and then translate the gene into its amino acid sequence,
d. What would happen to the gene if the adenosine mutates to a thymine where the arrow indicates?
3' TACTTTATTAGTCAATTGTCGTCAAGGCTAAAAATATGA 5'
What type of mutation is this?
Transcribed Image Text:Second letter
G
UUU
Phe
UUC
UAU
Tyr
UAC
UCU
UGU
Cys
UCC
UGC
Ser
UAA Stop
UGA Stop
UUA
Leu
UUG.
UCA
UCG
UAG Slop UGG Trp
CUU
CCU
CAU
His
CGU
CUC
Leu
CUA
CC
Arg
C
CAC
Pro
CAA
Gin
CAG
CCA
CGA
CUG.
CG
AAU
Asn
AAC
AGU
AUU
AUC le
ACU
Ser
ACC
AGC
The
ACA
AAA)
AGA
Arg
AGG
AUA
AUG Met
AAGLy
ACG
GUU
GCU
GAU)
Asp
GGU
GAC
GCC
Ala
GGC
Gly
GGA
GUC
Val
GUA
GCA
GAA
Glu
GAG
GUGJ
GCG
GGG
First letter
Thind letter
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- A. DNA Replication Construct a DNA with 15 base pairs. (Note that the first three nucleofides of the parent DNA (3' to 5') strand correspond to a start codon and its last three nucleotides correspond to a stop codon in its MRNA counterpart later on.) Write it down as follows: a. the sequence of parent DNA (template) 3' A C A TT 5' 3' Upon undergoing DNA replication, show what one daughter DNA molecule will look like. Write it down as follows: b. the sequence of DNA Daughter 1: 3' 5' 5' 3' C. the sequence of DNA Daughter 2: 3' 3' 5' in inarrow_forwardAssume that you are a RNA polymerase. Which strand is the template strand? -5 ↓ 3’-TCTTCGTGAGATGATATAAGAGTTATCCAGGTACCGGTAAACTGG-5’ 5’-AGAAGCACTCTACTATATTCTCAATAGGTCCATGGCCATTTGACC-3’arrow_forward32)An origin of replication is given below. Sequences of selected parental strand regions are given. The directionality of the bottom parental strand is indicated. Use the diagram to answer the corresponding questions. #2 *1 CTAAGCA ATCGAGG XXXXX XXXX 3' ICTAGTT 5' ge exon #3 a.) On the diagram, label the 5' and 3' end of the top strand of parental DNA b Draw arrows to indicate direction of DNA synthesis for each of the 4 daughter strands. e) For each daughter strand, specify if synthesis is continuous or discontinuous. d) On your diagram, label the 5' and 3' ends of the newly synthesized daughter strands e.) Which parental strands are the template for leading strand synthesis? (# 1- 4) Strand # Strand # f.) Which parental strands are the template for lagging strand synthesis? (# 1- 4) Strand # Strand # g.) What is the specific sequence of the primer required to start DNA replication ior strands 1 and 3? Assume the primer will bind to the location where the base sequence is given on…arrow_forward
- Label the diagram to the corresponding letterarrow_forwarda. Replicate this sense strand to create a double-stranded DNA helix. Write your answers in CAPS LOCK with NO SPACES between the nucleotides - e.g. ATGCCGAG..... TGAGGATGAAACTCACACCGGGGCGCAGTTTGGCACTTAGATTCTTGTACACGACCTAGTATAACACAGTT complementary strand: b. Using this DNA double helix, express the gene – i.e. determine the resulting polypeptide sequence by using the correct reading frame. Write your answers using the three letter abbreviation for each amino acid. polypeptide sequence: does the sense strand DNA sequence have 5’ and 3’ UTR sequences? 5'UTR = 3'UTR =arrow_forwardConsider the following segment of DNA:5′ GCTTCCCAA 3′3′ CGAAGGGTT 5′Assume that the top strand is the template strand usedby RNA polymerase.a. Draw the RNA transcribed.b. Label its 5′ and 3′ ends.c. Draw the corresponding amino acid chain.d. Label its amino and carboxyl ends.Repeat parts a through d, assuming the bottom strand tobe the template strand.arrow_forward
- On paper, replicate the following segment of DNA: 5’ A T C G G C T A C G T T C A C 3’ 3’ T A G C C G A T G C A A G T G 5’a. Show the direction of replication of the new strands and explainwhat causes lagging and leading strands to show different patternsof replication.b. Explain how this is semiconservative replication. Are the newstrands identical to the original segment of DNA?arrow_forwardA DNA antisense strand contains the following nucleotide base sequence: ACG TTT ATG GGT From this, what is the nucleotide sequence of the mRNA strand that is transcribed? Select one: a. UGC AAA UAC CCA b. TGC AAA TAC CCA c. ACG UUU AUG GGU d. AGC UUU AUC CCUarrow_forwardUsing the table of genetic code, choose the CORRECT protein sequence that will be produced from the following sense strand of DNA: 5'-AUG UCU GAC UAG TTG GAT CCC - 3' Second position First position (5' end) Third position (3' end) Phe Ser Tyr Cys U Phe Ser Тут Cys Leu Ser Stop Stop A. Leu Ser Stop Trp Leu Pro His Arg Leu Pro His Arg Leu Pro Gln Arg Leu Pro Gln Arg Ile Thr Asn Ser U Ile Thr Asn Ser Ile Thr Lys Arg A Met Thr Lys Arg Val Ala Asp Gly U Val Ala Asp Gly C Val Ala Glu Gly A Val Ala Glu Gly O a. Lys-His-Ala-Gly-Asn-Leu-Val O b. Val-Val-Ser-Pro-Leu-Asp-Thrarrow_forward
- Using the codon chart, if the DNA strand being described is AGG TCT GAT , the resulting amino acid would be whatarrow_forwardThe DNA sequence ATCAGCGCTGGC is part of a gene. how many amino acids are coded for by this message? 4. 8. 12 20 O Option 5arrow_forwardWhat is the sequence of the newly synthesize DNA segment if the template strand has the following sequence:3'-ATGGCCTATGCGAT-5'arrow_forward
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