8. Describe the steps involved in the process of genetic engineering via recombinant DNA technology. 9. Identify one specific example of how this process has been used. 10. Using the example provided in question 9, explain the rationale for the creation of this transgenic organism.

5b. When you mutagenize a diploid organism, chances are astronomically high that you will be creating a heterozygote on the spot. Why would your mutation be heterozygous and not homozygous?

1. Scientists were able to develop transgenic bacteria that can be used for mercury remediation.  This process involved combining genes for mercury resistance (so that the bacteria itself has protection) and mercury accumulation (thereby allowing the bacteria to sequester Hg for retrieval later on). They probably used the following techniques in creating the transgenic bacteria EXCEPT a. plasmids b. DNA ligase c. DNA polymerase d. restriction enzymes   2. Consider these two parents (Mom and Dad) and their son, Calvin.  (see pic attached). Which two members of this family would show the greatest difference in their DNA profile?

Place these events in the order they occur during mutagenesis: 1 1. The cell makes a repair error t 2. Damage to DNA by a mutagen 3. DNA repair pathways are used 1 4. A permanent change to the DNA sequence is made 1 5. Genetic variation is created in the population 1 6. A new allele is born!