10. The amino terminus of a wildtype enzyme in yeast has the amino acid sequence Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro A mutant, X, is found that contains an inactive form of the enzyme with the sequence 1_12_2021 Met-Gly-Asp-Tyr-Pro at the amino terminus and a wildtype sequence at the carboxyl terminus. A second mutant, Y, also lacks enzyme activity, but a full-length protein is not observed. Instead, mutant Y makes a short peptide containing just 3 amino acids. What mutations could account for these changes in mutant X and mutant Y? As part of your answer, show the sequences of the genes encoding the wildtype proteins and the 2 mutant proteins, and indicate in the wild type sequence where the mutations that gave rise to X and Y actually occurred. What is the sequence of the tripeptide produced by mutant Y? 4

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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10. The amino terminus of a wildtype enzyme in yeast has the amino acid sequence
Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro
A mutant, X, is found that contains an inactive form of the enzyme with the sequence
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Met-Gly-Asp-Tyr-Pro
at the amino terminus and a wildtype sequence at the carboxyl terminus. A second
mutant, Y, also lacks enzyme activity, but a full-length protein is not observed. Instead,
mutant Y makes a short peptide containing just 3 amino acids. What mutations could
account for these changes in mutant X and mutant Y? As part of your answer, show the
sequences of the genes encoding the wildtype proteins and the 2 mutant proteins, and
indicate in the wild type sequence where the mutations that gave rise to X and Y actually
occurred. What is the sequence of the tripeptide produced by mutant Y?
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Transcribed Image Text:Content → X # 3 -fleet01-xythos.content.blackboardcdn.com/blackboard.learn.xythos.prod/5a3683e2386d2/11826556?X-Blackboard-S3-Bucket-blackb... 1_12_2021 Bb 11826570 $ 10. The amino terminus of a wildtype enzyme in yeast has the amino acid sequence Met-Leu-His-Tyr-Met-Gly-Asp-Tyr-Pro A mutant, X, is found that contains an inactive form of the enzyme with the sequence 4 CO G Search or type URL X 4 | 7 | - 125% + | @ Met-Gly-Asp-Tyr-Pro at the amino terminus and a wildtype sequence at the carboxyl terminus. A second mutant, Y, also lacks enzyme activity, but a full-length protein is not observed. Instead, mutant Y makes a short peptide containing just 3 amino acids. What mutations could account for these changes in mutant X and mutant Y? As part of your answer, show the sequences of the genes encoding the wildtype proteins and the 2 mutant proteins, and indicate in the wild type sequence where the mutations that gave rise to X and Y actually occurred. What is the sequence of the tripeptide produced by mutant Y? % 5 Bb Genetics A 6 X & 7 ☆ Bb Genetics * 8 + - 9 O 4 O X *
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