Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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summarise the Concept of optimal conditions/ denaturing enzyme
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- The purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?arrow_forwardWhat are the concepts of specificity, competition, and saturation as they relate to enzymes. Include how enzymes show each property.arrow_forwardExplain the relationship between enzyme sensitivity and theadaptations microbes make to their environmentsarrow_forward
- When considering treatment options for SCID, enzyme replacement offers a permanent fix for patients while gen therapy offers short-term relief from symptoms True Falsearrow_forwardDefine these aspects of bioenergetics: couple reactions, allosteric enzymes, activation energyarrow_forwardThere is an enzyme inhibition mechanism that is not too detrimental when viewed from the size of Km, namely the inhibition mechanism.... A. non-competitiveB. non-competitive and uncompetitiveC. in-competitive and uncompetitiveD. in-competitiveE. uncompetitivearrow_forward
- You design an enzyme assay and choose a substrate concentration equal to the Km of the enzymatic reaction. You measure the rate of the reaction at this substrate concentration to be 75.0 μmol/min. Calculate the Vmax of this reaction in μmol/min. Express your answer with one decimal place. Your previous attempt suffered from low signal-to-noise ratio when you used a substrate concentration equal to the Km. So you decide to increase the substrate concentration to 0.45 mM. You remeasure the rate of the reaction at this new substrate concentration and find it to be 135.0 μmol/min. Using the same Vmax value found above, calculate the Km value of this enzymatic reaction. Express your answer in μM with one decimal place. Considering the previous two questions, what would be the rate of the reaction at a substrate concentration of 0.01 mM? Express your answer in μmol/min with one decimal place.arrow_forwardIn microbiology explain the relationship between pH and enzyme activity, be sure to include optimal pH range and denaturationarrow_forward
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