Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 9, Problem 11P
Interpretation Introduction
Interpretation:
The expected value for
Concept introduction:
The biological catalysts which lower the minimum amount of energy required by the reactants to convert into product are known as enzymes. An enzyme does not alter the potential energy of reactant and product. Carbonic anhydrase is an enzyme used in catalytic interconversion.
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I. Buffer Preparation
A researcher in Biochemistry is isolating α-glucosidase enzyme from malted wheat flour. The protocol requires the use of 2.00L of 0.225 M of lactate buffer with pH of 4.25. How will he/she prepare this buffer from 2.00 M lactic acid solution and solid sodium lactate (NaC3H5O3) The Ka of lactic acid is 1.38 x 10-4. [ Na=23.0, C=12.0, H=1.01, O=16.0 g/mol]
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