An explanation corresponding to the fact that in the conversion of His 64 to Ala, the mutant enzyme will cleave substrate B about 1000 times faster than substrate A is to be stated. Concept introduction: The biological catalysts which lower the minimum amount of energy required by the reactants to convert into a product are known as enzymes. An enzyme does not alter the potential energy of reactant and product. Proteolytic enzymes commonly known as proteases are used for the cleavage of protein.

Question

Need help. A protein X binds Ligand Y. The kon characterizing this binding is 1 x 106 M-1s-1, koff is 2 x 10-3 M-1s-1. In your in vitro X-Y binding experiment, at what concentration of Ligand Y half of the Protein X would be bound to Y. Assume that the binding is according to the Lock-and-key model

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Question

Chapter 9, Problem 2P

Interpretation Introduction

Interpretation:

An explanation corresponding to the fact that in the conversion of His 64 to Ala, the mutant enzyme will cleave substrate B about 1000 times faster than substrate A is to be stated.

Concept introduction:

The biological catalysts which lower the minimum amount of energy required by the reactants to convert into a product are known as enzymes. An enzyme does not alter the potential energy of reactant and product. Proteolytic enzymes commonly known as proteases are used for the cleavage of protein.

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