An explanation regarding the termination of reaction in which a restriction enzyme cleaves DNA by the addition of EDTA is to be stated. Concept introduction: The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the DNA sequence. These restriction enzymes are BamHI obtained from bacillus amyloliquifaciens, SmaI obtained from Serratia marcescens and many others.

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Chapter 9, Problem 12P

Interpretation Introduction

Interpretation:

An explanation regarding the termination of reaction in which a restriction enzyme cleaves DNA by the addition of EDTA is to be stated.

Concept introduction:

The restriction enzymes are also called as restriction endonucleases. The restriction endonucleases are used for the hydrolysis of the phosphodiester backbone present on the DNA sequence. These restriction enzymes are BamHI obtained from bacillus amyloliquifaciens, SmaI obtained from Serratia marcescens and many others.

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