The effect that occurs in chymotrypsin when Ser 189 is changed to Asp 189 is to be predicted. Concept introduction: The organic compounds that contain amine ( -NH 2 ) and carboxyl ( -COOH ) functional groups with a side chain ( -R ) are known as amino acids. Amino acids consist of both positive as well negative groups in their structure and are called as ampholytes. The small chain of amino acids that is connected with the help of the amide bonds is known as peptide. A peptide possesses the covalent bonds which are formed by the reaction of carboxyl group of one amino acid with the amino group of other amino acids. Proteolytic enzymes commonly known as proteases are used for the cleavage of protein. Chymotrypsin is an enzyme that is used for the cleavage of protein through hydrolysis reaction.

Question

Need help. A protein X binds Ligand Y. The kon characterizing this binding is 1 x 106 M-1s-1, koff is 2 x 10-3 M-1s-1. In your in vitro X-Y binding experiment, at what concentration of Ligand Y half of the Protein X would be bound to Y. Assume that the binding is according to the Lock-and-key model

Answer 1

Question

Chapter 9, Problem 4P

Interpretation Introduction

Interpretation:

The effect that occurs in chymotrypsin when Ser 189 is changed to Asp 189 is to be predicted.

Concept introduction:

The organic compounds that contain amine (-NH2) and carboxyl (-COOH) functional groups with a side chain (-R) are known as amino acids. Amino acids consist of both positive as well negative groups in their structure and are called as ampholytes. The small chain of amino acids that is connected with the help of the amide bonds is known as peptide. A peptide possesses the covalent bonds which are formed by the reaction of carboxyl group of one amino acid with the amino group of other amino acids.

Proteolytic enzymes commonly known as proteases are used for the cleavage of protein. Chymotrypsin is an enzyme that is used for the cleavage of protein through hydrolysis reaction.

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