Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 9, Problem 8P
Interpretation Introduction

Interpretation:

An explanation corresponding to the fact that the high concentration metal chelator EDTA treatment of carbonic anhydrase results in the loss of activity is to be stated.

Concept introduction:

The biological catalysts which lower the minimum amount of energy required by the reactants to convert into product are known as enzymes. An enzyme does not alter the potential energy of reactant and product. Carbonic anhydrase is an enzyme that is used as a catalytic for the interconversion between CO2, H2O and the carbonic acid dissociated ions.

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Instructions. Given each set of information which may include common name(s) and the reaction catalyzed, you are required to identify the main class of the specific enzyme described. Name: citryl-CoA synthetase Reaction: ATP + citrate + CoA = ADP + phosphate + (3S)-citryl-CoA Name: D-xylulose reductase Reaction: xylitol + NAD+ = D-xylulose + NADH + H+ Name: cellobiose phosphorylase Reaction: cellobiose phosphate = α-D-glucose 1-phosphate + D-glucose Name: carbonic anhydrase Reaction: H2CO3 = CO2 + H2O Other info: The enzyme catalyzes the reversible hydration of gaseous CO2 to carbonic acid, which dissociates to give hydrogencarbonate above neutral pH. Name: pantoate activating enzyme Reaction: ATP + (R)-pantoate = AMP + diphosphate + (R)-pantothenate.
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G. ENZYME CLASSIFICATION. Identify the main class of enzymes used to catalyzed the following reactions: 1. Lactate dehydrogenase: NADH+H NAD HC-OH CH3 CH Pynnte Lactate 2. Methylmalonyl-CoA mutase: CH CH SCOA CH,CH, SCOA coenzyme B12 COO COO methylmalonyl-CoA succinyl-CoA 3. Enolase: 0. H–Ċ–0–P–0- C-0–P-0- + H,0 HO–CH, CH 6 Phosphoenolpyruvate 2-Phosphoglycerate 4. Chymotrypsin: -0–CH,CH3 + H2O - RCOOH + HOCH,CH3 5. Pyruvate carboxylase: coo • co, • ATP + H,0 H-C-H . ADP + P, + 2H čoo CH, Pyruvate Oxaleacetate
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