Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 8.L2, Problem 8CT
8. At which site in the mitochondrion and chloroplast do the ATP molecules collect after being made by ATP synthases, and what is the advantage of this?
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9. Two nutrient solutions are maintained at the same pH. Actively respiring mitochondria are
isolated and placed into each of the two solutions. Oxygen gas is bubbled into one solution. The
other solution is depleted of available oxygen. Which of the following best explains why ATP
production is greater in the tube with oxygen than in the tube without oxygen?
(A) The rate of proton pumping across the inner mitochondrial membrane is lower in the sample
without oxygen.
(B) Electron transport is reduced in the absence of a plasma membrane.
(C) In the absence of oxygen, oxidative phosphorylation produces more ATP than does
fermentation.
(D) In the presence of oxygen, glycolysis produces more ATP than in the absence of oxygen.
9 .The energy of electron transport (by the ETC) serves to move protons (hydrogen ions, or H+) to the intermembrane space of the mitochondrion. How does this help the mitochondrion produce ATP?
a) This movement of protons sets up the electrochemical gradient that drives ATP synthesis in the mitochondrion.
b) The protons pick up electrons from the electron transport chain on their way through the inner mitochondrial membrane
c) The protons receive electrons from the NAD+ and FAD that were accepted in glycolysis ad the citric acid cycle
d) The protons are transferred to oxygen in an energy-releasing reaction
1. What would be the effect on ATP production during chemiosmosis and oxidative phorphorylation, if the pH within the inner membrane space of the mitochondrion were decreased, assuming no effect of pH on the structure of proteins in the membrane?
2. What would be the effect on production of ATP from photosynthesis, assuming no effect on enzyme/protein stability, if the pH of the chloroplast stroma were lowered?
Chapter 8 Solutions
Foundations in Microbiology
Ch. 8.1 - 1. Define metabolism and differentiate its two...Ch. 8.1 - Prob. 2ELOCh. 8.1 - 3. outline the prominent characteristics of...Ch. 8.1 - 4. Explain how enzymes lower the energy required...Ch. 8.1 - 5. Discuss enzyme structure, and interactions...Ch. 8.1 - 6. Describe the types of enzyme functions and...Ch. 8.1 - 7. Summarize key features of enzyme regulation.Ch. 8.1 - 1. Differentiate between catabolism and anabolism...Ch. 8.1 - 2. Describe 10 important biochemical properties of...Ch. 8.1 - 3. Describe the chemistry of enzymes, and explain...
Ch. 8.1 - 4. Show diagrammatically the interaction of...Ch. 8.1 - 5. Differentiate among the chemical composition...Ch. 8.1 - 6. Summarize the direct and indirect controls that...Ch. 8.2 - Prob. 8ELOCh. 8.2 - 9. Describe biological oxidation-reduction and...Ch. 8.2 - Prob. 10ELOCh. 8.2 - 7. Explain how oxidation of a substrate proceeds...Ch. 8.2 - 8. Refer to the blue redox equation for...Ch. 8.2 - 9. In the following redox pairs, which compound is...Ch. 8.2 - 10. a. Describe the roles played by ATP and NAD+...Ch. 8.2 - Prob. 11CYPCh. 8.2 - 12. What is meant by the concept of the “final...Ch. 8.3 - 11. Relate the main points of bioenergetics and...Ch. 8.3 - 12. Describe the main catabolic pathways and their...Ch. 8.3 - 13. Define glycolysis and explain its input and...Ch. 8.3 - Prob. 14ELOCh. 8.3 - 15. Describe the components of the respiratory...Ch. 8.3 - 16. Explain the chemiosmotic mechanism of ATP...Ch. 8.3 - 17. Summarize the results of aerobic respiration.Ch. 8.3 - Prob. 18ELOCh. 8.3 - 13. Describe the basic energy strategies of...Ch. 8.3 - Prob. 14CYPCh. 8.3 - 15. Outline the basic steps in glycolysis,...Ch. 8.3 - Prob. 16CYPCh. 8.3 - 17. What is the fate of NADH in a fermentative...Ch. 8.3 - 18. Summarize the chemiosmotic theory of ATP...Ch. 8.3 - 19. Haw many ATPs could theoretically be formed...Ch. 8.3 - Prob. 20CYPCh. 8.3 - 21. Name the sources of oxygen in bacteria that...Ch. 8.3 - 22. What are the final electron acceptors in...Ch. 8.3 - Prob. 23CYPCh. 8.4 - 19. Explain what is meant by the term fermentation...Ch. 8.4 - 20. Describe some of the processes of fermentation...Ch. 8.4 - 24. What adaptive advantages does a fermentative...Ch. 8.4 - 25. Describe three patterns of fermentation...Ch. 8.5 - 21. Explain how cells perform anabolic functions...Ch. 8.5 - 22. Identify major pathways where molecules can be...Ch. 8.5 - 23. Briefly describe several mechanisms in...Ch. 8.5 - 26. What is meant by amphibolism, and what are its...Ch. 8.5 - Prob. 27CYPCh. 8.5 - 28. Which macromolecules are synthesized by...Ch. 8.6 - 24. Outline the general reactions of...Ch. 8.6 - 25. Describe the pigment systems and how they...Ch. 8.6 - 26. Describe the main events in the...Ch. 8.6 - 27. Describe the main events in the...Ch. 8.6 - 29. Indicate whether each of the following is...Ch. 8.6 - Prob. 30CYPCh. 8.6 - 31. What are the functions of chlorophyll and the...Ch. 8.6 - Prob. 32CYPCh. 8.6 - 33. Compare oxygenic with nonoxygenic...Ch. 8.L1 - 1. ______ is another term for biosynthesis. a....Ch. 8.L1 - Prob. 2MCQCh. 8.L1 - 3. An enzyme ___________ the activation energy...Ch. 8.L1 - 4. An enzyme a. becomes part of the final products...Ch. 8.L1 - 5. An apoenzyme is where the ___________ is...Ch. 8.L1 - 6. Many coenzymes contain a. metals b. vitamins c....Ch. 8.L1 - 7. To digest cellulose in its environment, a...Ch. 8.L1 - 8. Energy in biological systems is primarily a....Ch. 8.L1 - 9. Energy is carried from catabolic to anabolic...Ch. 8.L1 - 10. Exergonic reactions a. release potential...Ch. 8.L1 - Prob. 11MCQCh. 8.L1 - Prob. 12MCQCh. 8.L1 - Prob. 13MCQCh. 8.L1 - 14. Fermentation of a glucose molecule has the...Ch. 8.L1 - Prob. 15MCQCh. 8.L1 - Prob. 16MCQCh. 8.L1 - 17. The FADH2 formed during the Krebs cycle enters...Ch. 8.L1 - 18. The proton motive force is the result of a....Ch. 8.L1 - Prob. 19MCQCh. 8.L1 - Prob. 20MCQCh. 8.L1 - 21. The oxygen produced by photosynthesis comes...Ch. 8.L1 - Prob. 22MCQCh. 8.L1 - Prob. 1CSRCh. 8.L1 - Prob. 2CSRCh. 8.L1 - Prob. 3CSRCh. 8.L1 - Prob. 1WCCh. 8.L1 - 2. Give the general name of the enzyme a. converts...Ch. 8.L1 - 3. Explain what is unique about the actions of ATP...Ch. 8.L1 - Prob. 4WCCh. 8.L1 - 5. Describe four requirements required for...Ch. 8.L1 - Prob. 6WCCh. 8.L1 - Prob. 7WCCh. 8.L1 - Prob. 8WCCh. 8.L2 - 1. Use the following graph to diagram the...Ch. 8.L2 - 2. Explain what is meant by the “biochemical...Ch. 8.L2 - 3. Explain how it is possible for certain microbes...Ch. 8.L2 - 4. Suggest the advantages of having metabolic...Ch. 8.L2 - 5. Two steps in glycolysis are catalyzed by...Ch. 8.L2 - 6. Beer production requires an early period of...Ch. 8.L2 - 7. What would be the expected pHs of the matrix...Ch. 8.L2 - 8. At which site in the mitochondrion and...Ch. 8.L2 - Prob. 9CTCh. 8.L2 - Prob. 10CTCh. 8.L2 - 1. From chapter 7. figure 7.11 (reproduced below)....Ch. 8.L2 - 2. Look at the two figure parts (a) and (b) from...
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- 6. The lens of the eye is the light refracting medium of the eye, and it has no mitochondria. Glucose is used as an energy source in the lens. Which metabolic pathway of glucose supplics energy to the crystalline eye lens? To answer the question: a) write a diagram of the metabolic pathway that provides the eye's lens with ATP, specify enzymes and coenzymes; b) mark the reactions associated with the ATP consumption and synthesis, calculate the ATP yield during the process; c) specify the mechanisms of ATP synthesis in the process; d) name the tissues and cells in which the ATP synthesis is the same as in the lens; e) write the dehydrogenation reaction and the reaction of final product formation occurring in the process; 6.3. Gluconeogenesis. Regulation of Glycolysis and Gluconeogenesis 271 ) indicate the fate of the end product of the process and the consequences of its accumulation.arrow_forward17. The process of cellular respiration in mitochondria generates which molecule that is used as the main energy store in cells?arrow_forward10. In ATP synthase, the ____ subunit is the site of ATP synthesis while the ____ subunit forms the proton channel through the inner mitochondrial membrane.arrow_forward
- 18. The figures below illustrate the similarities between ATP synthesis in mitochondria and chloroplasts. Mitochondrion NADH H,0 Δ H*. ATP ADP P; Inner Mitochondrial Membrane Chloroplast H,O NADP+ NADPH H* H+- ATP ADP Thylakoid Membrane A A Hydrogen ion O Electron The figures can best assist in answering which of the following questions? (A) Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules? (B) What are the sources of energy that drive mitochondrial and chloroplast electron transport systems? (C) What is the optimal temperature at which ATP synthase chemically converts ADP and a phosphate group into one molecule of ATP? (D) What is the evolutionary relationship between the ATP synthase in mitochondria and the ATP synthase in chloroplasts? A +B + energy → AB 19. Which of the following best characterizes the reaction represented above? (A) Hydrolysis (B) Catabolism (C) Oxidationreduction (D) Exergonic reaction (E) Endergonic reactionarrow_forward17. What is the Net total of ATP made during Electron Transport?18. What is the Net total of ATP made during the entire process of Cellular Respiration?19. What are the supermolecules NADH and FADH2 needed for?20. Which of the 3 processes will NADH and FADH2 be utilized?arrow_forward12. Use Figure 4 for questions a-c below. 114 ATP synthase matrix intermembrane space Figure 4: Sketch of a mitochondrion https://upload.wikimedia.org/wikipedia/commons/1/1a/Schema mitochondrion basic.svg Bionet, CC BY-SA 4.0 , via Wikimedia Commons a. Add to the sketch to show a H* concentration gradient in an active mitochondrion. (Sketch more H* in the appropriate space. Sketch less H* in the appropriate space.) 4+ b. Sketch an arrow to represent the direction that protons flow (via diffusion) through ATP synthase. c. If the concentration of protons was equal on each side of the membrane, how would that affect the flow of protons through ATP synthase? How would that affect ATP production from glucose?arrow_forward
- 6. The lens of the eye si the light refracting medium of the eye, and ti has no mitochondria. Glucose is used sa an energy source ni the lens. Which metabolic pathway of glucose supplies energy to the crystalline eye lens? To answer the question: a) write a diagram of the metabolic pathway that provides the eye's lens with ATP, specify enzymes and coenzymes; b) mark the reactions associated with the ATP consumption and synthesis, calculate the ATP yield during the process; )c specify the mechanisms ofATP synthesis ni the process; d)name the tissues and cells in which the ATP synthesis is the same as in hte lens; e) writethe dehydrogenation reaction and the reactionoffinal product formation occurring in the process;arrow_forward18. Photosynthesis and aerobic cellular respiration both rely on electron transport chains to generate ATP. Which of the following does not correctly identify similarities and differences in the ETC of these processes? A. Electrons delivered to the ETC are used to generate a proton gradient across the membrane B. In photosynthesis, the facilitated diffusion of protons across the membrane generates ATP and glucose molecules; in cellular respiration, this process generates ATP C. In photosynthesis, electrons are delivered to the ETC by NADPH; in cellular respiration, electrons are delivered to the ETC by NADH and FADH2 D. In prokaryotes, active transport moves protons across the cell's plasma membrane during photosynthesis and cellular respirationarrow_forward10. ATP synthase is a key enzyme of mitochondrial energy conversion. Mitochondrial ATP synthase deficiency is due to a mutation in a gene important for the formation of a subunit in the ATP synthase complex, seen in the illustration below. Scientists could use cells with this gene mutation to investigate which of the following questions? (LS1-7) * Inner mitochondrial membrane PYRUVATE GLYCOLYSIS ODATION ACO CYCLE OXIDATIVE PHOSPHORYL- ATION ATP synthase Protein complex of electron Intermembrane- space carriers III Inner mitochondrial membrane FADH, FAD 2 H* + ½02 H,0 NAD NADH (carrying electrons from food) ADP + O АТР Mitochondrial matrix Oxidative phosphorylation What effect does the mutation have on the movement of electrons between the electron carriers of the electron transport chain? What effect does the mutation have on the amount of ATP synthesized during cellular respiration? What effect does the mutation have on the number of protons pumped into the intermembrane space of the…arrow_forward
- 1. A toxin that inhibits the establishment of the proton gradient across the inner mitochondrial membrane would cause the following result: ATP synthase would be unable to produce ATP. Substrate-level phosphorylation would be inhibited. Pyruvate would be unable to enter the TCA cycle. NADH would be oxidized. Fermentation could not occur.arrow_forward3. The following diagram shows some of the metabolic processes taking place in a plant cell. a) Identify process Y and state the name of the organelle where it takes place in a plant cell. b) Process X uses oxygen and produces ATP. Identify process X. c) With reference do the diagram, identify one example of catabolismarrow_forward18.Substrate-level phosphorylation (A) Energy released from ATP hydrolysis is used to phosphorylate the substrate (B) Energy released from the chemical reaction is used to directly phosphorylate ADP (C) Energy released from ATP hydrolysis is used to phosphorylate ADP (D) Energy released from the chemical reaction is used to directly reduce NAD+arrow_forward
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