Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 10MC
Summary Introduction
Introduction:
Microarray technique is the hybridization of genes present on slides with
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DNA can be separated based on fragment size using ________.
a.
restriction enzymes
b.
gel electrophoresis
c.
PCR
____ cut(s) DNA molecules at specific sites.
a.
DNA probes
b.
Restriction enzymes
c.
DNA polymerase
d.
DNA ligase
_____ can correct a genetic defect in an individual. a. Cloning vectors c. Sequencing b. Gene therapy d. Electrophoresis
Chapter 8 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Prob. 7MC
Ch. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Prob. 1MTFCh. 8 - Prob. 2MTFCh. 8 - Prob. 3MTFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5MTFCh. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 6CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- _____ cut(s) DNA molecules at specific sites. a. DNA polymerase c. Restriction enzymes b. DNA probes d. Reverse transcriptasearrow_forwardChoose the combination of answers that most accurately completes the statement.For which of the following would a nucleic acid probe not be used? a. locating a gene on a chromosome b. developing a Southern blot c. identifying a microorganism d. constructing a recombinant plasmidarrow_forwardEnzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forward
- RFLP analysis involves the following, except ________ . a. more than one restriction sites b.gene therapy c.intercalation of dye d.electrophoresis e.restrictionarrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forwardLinkage analysis ________. A. is used to create a physical map B. Involves breaking and rejoining of DNA artificially (incorrect) C. Requires radiation hybrid mapping D. Is based on natural recombination process thank you in advance for your help!arrow_forward
- Plasmid vectors have the following characteristics, except _________ . a. MCS b. expression c. transfection d. single stranded e. cloningarrow_forwardFeatures of restriction enzymes include the following, except __________ . a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNAarrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forward
- Which of the following is involved in recombinant DNA technology? Explain. a. DNA polymerase b. DNA probes c. Restrition enzymes d. Reverse transcriptasearrow_forwardWe want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNAarrow_forwardThe other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forward
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