Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 8, Problem 5MC
Summary Introduction
Introduction:
Recombinant
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Which lab technique can you use to verify?
a.Nucleic Acid Purification
b. PCR
c.SNP Variation
What repair system will have an issue functioning properly if DNA glycosylase loses its function?
A. Photoreactivation repair
B. Base excision repair
C. Nucleotide excision repair
D. SOS repair
What is used to cut the DNA in panels 1 and 2?
a.
Restriction enzymes
b.
DNA polymerases
c.
DNA ligases
d.
RNA polymerases
e.
Reverse transcriptase
Chapter 8 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Prob. 7MC
Ch. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Prob. 1MTFCh. 8 - Prob. 2MTFCh. 8 - Prob. 3MTFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5MTFCh. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 6CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- which process releases the DNA into the ionic medium? a. homogenization b. deproteination c. precipitation d. all are correctarrow_forwardWhich type of mutation produces the same protein despite a change in the DNA? A. nonsense B. missense C. silent D. frameshiftarrow_forwardDNA polymerases that corrects errors and mismatches in DNA is called? A. Post replication repair B. Base excision repair C. Nucleotide excision repair D. Proofreadingarrow_forward
- Choose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? a.DNA ligase seals any gaps remaining between bases of new DNA. b.Primers base-pair with the exposed single DNA strands. c.Repair enzymes correct potential mutations in the DNA sequence. d.Enzymes unwind and separate the two strands of DNA.arrow_forward
- What is the role of Sodum Dodecyl Sulffate in DNA isolation? A. Precipitate DNA B Make the solution more viscous C Destroy the cell membrane D Maintain ionic strengtharrow_forwardA mutation in which of these proteins will lead to increased mutations in all daughter cells? A. helicase B. topoisomerase C. ligase D. DNA polymerasearrow_forwardWhat is the role of Sucrose in DNA isolation? A.Cell Lysis B. Maintain osmotic pressure C. Maintain ionic strength D. Precipitate proteinsarrow_forward
- Which DNA fragment will be closest to the top (negative pole) of anelectrophoretic gel?a. 450 bp b. 3,560 bp c. 5 kb d. 1,500 bparrow_forwardWhich step follows the assembly of new DNA strands by DNA polymerase? A. Enzymes unwind and separate the two strands of DNA. B. Repair enzymes correct potential mutations in the DNA sequence. C. Primers base-pair with the exposed single DNA strands. D. DNA ligase seals any gaps remaining between bases of new DNA.arrow_forwardWhat is meant by the term DNA replication? a. synthesis of nucleotides b. cell division c. interpretation of the genetic code d. the exact copying of the DNA code into two new moleculesarrow_forward
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