Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 1CM
Using the following terms, fill in the following concept map that describes recombinant DNA technology. You can also complete this and other concept maps online by going to the MasteringMicrobiology Study Area.
Blunt ends
DNA probes
Electroporation
Fluorescent
Injection
Mutagens
Plasmids
Protoplast fusion
Radioactive
Restriction enzymes
Reverse transcriptase
Southern biota
Transposons
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Chapter 8 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Prob. 7MC
Ch. 8 - Prob. 9MCCh. 8 - Prob. 10MCCh. 8 - Prob. 1MTFCh. 8 - Prob. 2MTFCh. 8 - Prob. 3MTFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5MTFCh. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 6CTCh. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...
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- For each of the following, give a brief description of the purpose and give an application Restriction enzymes Plasmids Gel Electrophoresis PCRarrow_forwardRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomearrow_forwardDefine the following terms: Plasmid Restriction Enzyme Standard Curvearrow_forward
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- You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a test group of strawberry plants. Select the events you would use to genetically engineer these plants. Insert the ice gene into the T-DNA region of a Ti plasmid; transform Agrobacterium tumefaciens with this plasmid; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. Insert the ice gene into the T-DNA region of the Agrobacterium tumefaciens chromosome; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. O Insert the ice gene into a Ti transposon; Introduce the transposon into Agrobacterium tumefaciens Ti plasmid: Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens: Select plant cells that have taken up the ice gene. Insert the ice gene into a Ti bacteriophage; transduce Agrobacterium tumefaciens with this…arrow_forwardWhat are plasmids and vectors how are they used in cloning and research? Write the answer in following headings What are plasmids What are vectors Plasmids in cloning and plasmids in research Vectors in cloning and vectors in research Write an answer of about 2 to 3 pagesarrow_forwardWhat are plasmids and vectors how are they used in cloning and research? Write the answer in following heading: What are plasmids ,What are vectors Plasmids in cloning and plasmids in research Vectors in cloning and vectors in research. Write an answer of about 2 to 3 pagesarrow_forward
- DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNasesarrow_forward________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymesarrow_forwardThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1. T7 RNA polymerase 2. Reverse transcriptase 3. EcoRIarrow_forward
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