Concept explainers
To review:
The expected pathway that is followed from the inside to the outside of the cell by:
a) a lysosomal enzyme,
b) a protein, which is excreted from the cell.
Introduction:
The motion of proteins is followed within a cell with the help of a pulse-chase experiment, in which the proteins are tagged during their synthesis. The tagging is done using radioactive isotope (radio-labeled amino acid), known as pulse, and the cells are monitored for protein synthesis for the certain time period.
The proteins incorporate the isotope and are labeled. Their location is then detected by isolating and separating the cell organelles and checking for radioactivity. This experiment is used to study various proteins such as protein kinases and ubiquitin.
Want to see the full answer?
Check out a sample textbook solutionChapter 5 Solutions
Life: The Science of Biology
- Protein structure and function: a) Name two common post-translational modifications of proteins in the cell that will affect their structure/function. b) What are prions? Briefly describe their structure and function. C) Explain the principles of protein folding and significance of urea and Bmercaptoethanol in the experimental procedures addressing this question.arrow_forwardProteinase K should be used as the first step of DNA purification (before applying phenol CIA mixture) to remove most proteins. Proteinase K alone cannot remove all proteins from the lysate. Explain why the Proteinase K enzyme cannot remove ALL proteins from the cell lysate.arrow_forwardProtein transfer to a membrane from a developed electrophoretic gel can be performed under which of the following conditions?arrow_forward
- How might the technique of affinity chromatography (see animation) be used to purify lectins?arrow_forwardOne of the blocking solutions that is traditionally used is non-fat milk, but this is not recommended for studies of phosphoproteins. Why?arrow_forwardProteoglycan aggregates in tissues form hydrated, viscousgels. Can you think of any obvious mechanical reason whytheir capacity to form gels is important to cell function?[Hint: Liquid water is virtually incompressible.]arrow_forward
- The monod model relates bacterial growth to substrate utilization. (i) The relationship of growth-limiting substrate utilization to bacterial growth is what is at the heart of the model. (ii) The mathematical equations of the model can vary in complexity depending on the given conditions (iii) Endogenous decay is the only mechanisms of cell death that is important enough to warrant representation in the monod model. Group of answer choices 1.Only (i) and (ii) 2.Only (i) and (iii) are correct 3.(i), (ii) and (iii) are all correctarrow_forwardA different Fab fragment binds to lysozyme with a dissociation constant of Kd=10-6 M. A 1 nM (10-9 M) solution of lysozyme is treated with increasing concentrations of this Fab fragment. At what concentration of added Fab will half of the lysozyme be bound to this Fab?arrow_forwardUsing the experimental results, describe the pathway that secretary proteins take from their synthesis to their release from the cell.arrow_forward
- Certain cellular components appear to move bidirectionally on microtubules. Describe how this is possible given that microtubule orientation is fixed by the MTOC.arrow_forwardThe allosteric regulation of ATCase by CTP is an example of: 1. Negative homotropic allostery 2. Positive homotropic allostery 3. Negative heterotropic allostery 4. Positive heterotropic allosteryarrow_forwardWhat is the expected outcome of the ion exchange chromatography using lysozyme as outlined above? Will the protein be separated successfully or not using the materials stated in the above? Provide a brief explanation to your reasoning please answer correctly, not written assigmentarrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education