Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 5, Problem 9P
A mutant form of polypeptide hormone angiotensin Il has the amino acid composition (Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val)
The following observations are made.
• Trypsin yields a dipeptide containing Asp and Arg and a hexapeptide with all the rest.
• Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro and a hexapeptide containing all others.
• Chymotrypsin cleaves the hormone into two tetrapeptides, of composition (Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro)
• The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase
What is the sequence of angiotensin II?
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A mutant form of polypeptide hormone angiotensin II has the amino acidcomposition (Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val)The following observations are made:• Trypsin yields a dipeptide containing Asp and Arg and a hexapeptide withall the rest.• Cyanogen bromide cleavage yields a dipeptide containing Phe and Proand a hexapeptide containing all the others.• Chymotrypsin cleaves the hormone into two tetrapeptides, of composition (Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro)• The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase.What is the sequence of angiotensin II?
H
CH₂
H₂C
HC-CH3
CH₂
H
H₂C
(S)
H₂C
H
CH₂
CH₂
CH₂
NH
O
C NH
NH₂
a) Which of the following statements about
this peptide are correct?
Group of answer choices
Treatment of this peptide with trypsin
generates two products.
This peptide is a substrate for
carboxypeptidase A
Treatment of this peptide with cyanogen
bromide generates a pentapeptide and a
tripeptide.
Treatment of this peptide with chymotrypsin
generates three products.
Treatment of this peptide with elastase
generates 2 products.
None of the above statements are correct.
b) What is the sequence of this peptide using
one letter abbreviations?
c) What is the pH which would correspond to
the ionization of the peptide as drawn above?
1, 5, 7, 10, 14
A mutant form of polypeptide hormone has the following amino acid composition:
Asp, Arg, lle, Met, Phe, Pro, Tyr, Val
The following observations are made:
Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide with all the rest.
Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro, and a hexapeptide containing all the others.
Chymotrypsin cleaves the hormone into two tetrapeptides with the following composition: (Asp, Arg, Tyr, Val) and (lle, Met Phe, Pro)
What is the amino acid sequence of this polypeptide?
Chapter 5 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 5 - Prob. 1PCh. 5 - Draw the structure of the peptide DTLH, showing...Ch. 5 - Prob. 3PCh. 5 - Prob. 4PCh. 5 - Prob. 5PCh. 5 - Prob. 6PCh. 5 - Prob. 7PCh. 5 - Given the following peptide SEPIMAPVEYPK a....Ch. 5 - A mutant form of polypeptide hormone angiotensin...Ch. 5 - Prob. 10P
Ch. 5 - Prob. 11PCh. 5 - a. Write a possible sequence for an mRNA segment...Ch. 5 - 13. Assume the following portion of an mRNA Find a...Ch. 5 - Prob. 14PCh. 5 - Prob. 15PCh. 5 - Prob. 16PCh. 5 - Prob. 17PCh. 5 - Prob. 18PCh. 5 - You are a summer intern in a clinical hematology...Ch. 5 - Prob. 20PCh. 5 - Despite the fact that many peptides have critical...Ch. 5 - Based on the information in Figure 5.17, which...Ch. 5 - If you want to purify a DNA-binding protein from a...
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