Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 12P
a. Write a possible sequence for an mRNA segment coding for apamine.
b. Do you think apamine is synthesized in the form shown in Problem 11, or is it more likely a product of proteolytic cleavage of a larger peptide? Explain.
11. Apamine is a small protein toxin present in the venom of the honeybee. It has the sequence
CNCKAPETALCARRCQQH
a. If apamine does not react with iodoacetate (see Tools of Biochemistry 5B), then how many disulfide bonds are present?
b. If trypsin cleavage gave two peptides, then where is(are) the S-S bond(s)?
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A. What amino acid sequence is encoded by the codon sequence AUAAUGGUAACGGUU?
B. Suppose the codon sequence AGACACUCUAUUAAA
has a single base pair mutation to AGACACUCUUUUAAA.
If the old protein sequence was Arg-His-Ser-Ile-Lys, what will be the new sequence encoded by the mutant gene?
(a) Write a possible sequence for an mRNA segment coding for apamine.(b) Do you think apamine is synthesized in the form, or is it more likely a product of proteolytic cleavage of a larger peptide? Explain.
. Why is a nonsense suppressor tRNATyr, even though ithas a mutant anticodon that cannot recognize a tyrosinecodon, charged with tyrosine by Tyr tRNA synthetase?
Chapter 5 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 5 - Prob. 1PCh. 5 - Draw the structure of the peptide DTLH, showing...Ch. 5 - Prob. 3PCh. 5 - Prob. 4PCh. 5 - Prob. 5PCh. 5 - Prob. 6PCh. 5 - Prob. 7PCh. 5 - Given the following peptide SEPIMAPVEYPK a....Ch. 5 - A mutant form of polypeptide hormone angiotensin...Ch. 5 - Prob. 10P
Ch. 5 - Prob. 11PCh. 5 - a. Write a possible sequence for an mRNA segment...Ch. 5 - 13. Assume the following portion of an mRNA Find a...Ch. 5 - Prob. 14PCh. 5 - Prob. 15PCh. 5 - Prob. 16PCh. 5 - Prob. 17PCh. 5 - Prob. 18PCh. 5 - You are a summer intern in a clinical hematology...Ch. 5 - Prob. 20PCh. 5 - Despite the fact that many peptides have critical...Ch. 5 - Based on the information in Figure 5.17, which...Ch. 5 - If you want to purify a DNA-binding protein from a...
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