Snake venom contains many hydrolase enzymes, including several serine proteases. One such protease (present in high concentrations in snake venom) mimics the action of thrombin to activate fibrin. The venom protease cleaves peptide bonds with a specificity for basic, positively charged amino acids. This enzyme is incubated with a substrate peptide with the sequence SNPDCKVALYMTW. Draw a mechanism for the peptide hydrolysis of this specific peptide catalyzed by this snake venom protease. Include the structure of the active site amino acids of the enzyme and the substrate peptide group (including the full structure of the 2 specific amino acids that contribute to the scissile peptide group in the substrate) in your mechanism. Use the curved arrow convention to illustrate the flow of electrons.
Snake venom contains many hydrolase enzymes, including several serine
proteases. One such protease (present in high concentrations in snake venom) mimics the
action of thrombin to activate fibrin. The venom protease cleaves peptide bonds with a
specificity for basic, positively charged amino acids. This enzyme is incubated with a substrate
peptide with the sequence SNPDCKVALYMTW. Draw a mechanism for the peptide hydrolysis
of this specific peptide catalyzed by this snake venom protease. Include the structure of the
active site amino acids of the enzyme and the substrate peptide group (including the full
structure of the 2 specific amino acids that contribute to the scissile peptide group in the
substrate) in your mechanism. Use the curved arrow convention to illustrate the flow of
electrons.
Step by step
Solved in 2 steps with 1 images
