Explanation The sequence of α -melanotropin is as follows, Ser − Tyr − Ser − Met − Glu − His − Phe − Arg − Trp − Gly − Lys − Pro-Val This peptide sequence has following charged amino acids, namely Amino acid pKa # Residues N-term 7.9 1 C-term 3.8 1 Arg 12.5 1 Glu 4.2 1 His 6 1 Try 10.1 1 Lys 10 1 The formula of charge calculation (amino terminal and amino side-chain with the amino group) at different pH for is as follows: ( T o t a l r e s i d u e s ) × ( 10 p K a − p H 10 p K a − p H + 1 ) The formula of charge calculation (Carboxylic terminal and amino side-chain with the Carboxylic group) at different pH for is as follows: ( − T o t a l r e s i d u e s ) × ( 10 − ( p K a − p H ) 10 − ( p K a − p H ) + 1 ) The charge contribution by N-terminal from pH 1-14 is as follows: N-terminal charge at pH -1: ( 1 ) × ( 10 7.9 − 1 10 7.9 − 1 + 1 ) = 1 N-terminal charge at pH 2: ( 1 ) × ( 10 7.9 − 2 10 7.9 − 2 + 1 ) = 0.9 ≈ 1 N-terminal charge at pH 3: ( 1 ) × ( 10 7.9 − 3 10 7.9 − 3 + 1 ) = 0.99 ≈ 1 N-terminal charge at pH 4: ( 1 ) × ( 10 7.9 − 4 10 7.9 − 4 + 1 ) = 0.99 ≈ 1 N-terminal charges at pH 5: ( 1 ) × ( 10 7.9 − 5 10 7.9 − 2 + 1 ) = 1 N-terminal charges at pH 6: ( 1 ) × ( 10 7.9 − 6 10 7.9 − 6 + 1 ) = 0.99 N-terminal charges at pH 7: ( 1 ) × ( 10 7.9 − 7 10 7.9 − 7 + 1 ) = 0.89 N-terminal charges at pH 8: ( 1 ) × ( 10 7.9 − 8 10 7.9 − 8 + 1 ) = 0.44 N-terminal charges at pH 9: ( 1 ) × ( 10 7.9 − 9 10 7.9 − 9 + 1 ) = 0.07 N-terminal charges at pH 10: ( 1 ) × ( 10 7.9 − 10 10 7.9 − 10 + 1 ) = 0.01 N-terminal charges at pH 11: ( 1 ) × ( 10 7.9 − 11 10 7.9 − 11 + 1 ) = 0.00 N-terminal charges at pH 12: ( 1 ) × ( 10 7.9 − 12 10 7.9 − 12 + 1 ) = 0.00 N-terminal charges at pH 13: ( 1 ) × ( 10 7.9 − 13 10 7.9 − 13 + 1 ) = 0.00 N-terminal charges at pH 14: ( 1 ) × ( 10 7... Interpretation Introduction (b) Interpretation: Calculate the charge on the a-melanotropin at pH 11, 5 and 1. Introduction: The net charge on the amino acid or the protein can be extrapolated by the titration graph. The net charge on the protein or any amino acid sequence depends on the charge on all the amino acid residue present in the chain. Interpretation Introduction (c) Interpretation: The calculate the isoelectric potential. Introduction: The isoelectric point (pl) is the pH at which the molecule has net zero charge.

Biochemistry: Concepts and Connections (2nd Edition)

2nd Edition

ISBN: 9780134641621

Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews

Publisher: PEARSON

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Question

Chapter 5, Problem 5P

Interpretation Introduction

(a) Interpretation:

To determine the titration diagram and to find the charge and isoelectric point of a-melanotropin.

Introduction:

The titration curve of the amino acids can be used to determine the isoelectric point of the amino acids. The isoelectric point is the point, at which the net charge on the amino acids becomes zero.

Interpretation Introduction

(b) Interpretation:

Calculate the charge on the a-melanotropin at pH 11, 5 and 1.

Introduction:

The net charge on the amino acid or the protein can be extrapolated by the titration graph. The net charge on the protein or any amino acid sequence depends on the charge on all the amino acid residue present in the chain.

Interpretation Introduction

(c) Interpretation:

The calculate the isoelectric potential.

Introduction:

The isoelectric point (pl) is the pH at which the molecule has net zero charge.

Expert Solution & Answer

Chapter 5 Solutions

Biochemistry: Concepts and Connections (2nd Edition)

Ch. 5 - Prob. 1P Ch. 5 - Draw the structure of the peptide DTLH, showing...Ch. 5 - Prob. 3P Ch. 5 - Prob. 4P Ch. 5 - Prob. 5P Ch. 5 - Prob. 6P Ch. 5 - Prob. 7P Ch. 5 - Given the following peptide SEPIMAPVEYPK a....Ch. 5 - A mutant form of polypeptide hormone angiotensin...Ch. 5 - Prob. 10P

Ch. 5 - Prob. 11P Ch. 5 - a. Write a possible sequence for an mRNA segment...Ch. 5 - 13. Assume the following portion of an mRNA Find a...Ch. 5 - Prob. 14P Ch. 5 - Prob. 15P Ch. 5 - Prob. 16P Ch. 5 - Prob. 17P Ch. 5 - Prob. 18P Ch. 5 - You are a summer intern in a clinical hematology...Ch. 5 - Prob. 20P Ch. 5 - Despite the fact that many peptides have critical...Ch. 5 - Based on the information in Figure 5.17, which...Ch. 5 - If you want to purify a DNA-binding protein from a...

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